Jul 1, 1992

Profiling assay for lipoxygenase products of linoleic and arachidonic acid by gas chromatography-mass spectrometry

Analytical Biochemistry
W D LehmannG Fürstenberger

Abstract

A method for determination of the lipoxygenase products of linoleic acid (9- and 13-hydroxyoctadecadienoic acid; 9-HODE, 13-HODE) and of arachidonic acid (5-, 8-, 9-, 11-, 12-, and 15-hydroxyeicosatetraenoic acid; 5-, 8-, 9-, 11-, 12-, and 15-HETE) is described. The method combines solid-phase extraction, derivatization to the corresponding fully hydrogenated methylester/trimethylsilylether derivatives and capillary gas chromatography coupled with electron impact mass spectrometry. Each regioisomeric HODE and HETE shows a unique pair of mass spectrometric fragment ions originating from fission of the fatty acid carbon chain at the hydroxylated position. The carboxyl-terminal fragment is used for quantification relative to a carboxyl-18O2-labeled analogue added as internal standard and the methyl-terminal fragment is monitored for confirmation. The assay can be extended for quantification of the complete hydroxylation profile of linoleic and arachidonic acid. Applications of this assay are demonstrated for the quantification of HODEs and HETEs in normal, hyperplastic, and neoplastic mouse epidermis. In mouse epidermis papilloma, the tissue levels of 8- and 12-HETE were found to be increased by one to two orders of magnitude comp...Continue Reading

Mentioned in this Paper

Malignant Neoplasm of Skin
Derivatives
Arachidonic Acid
Analog
Gas Chromatography-Mass Spectrometry
Lipoxygenase-2
12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
Linoleic Acids, Conjugated
Linoleic Acids
Lipoxygenase

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