Profiling Atlantic salmon B cell populations: CpG-mediated TLR-ligation enhances IgM secretion and modulates immune gene expression

Scientific Reports
Shiferaw JenberieJorunn B Jørgensen

Abstract

While TLR-activated pathways are key regulators of B cell responses in mammals, their impact on teleost B cells are scarcely addressed. Here, the potential of Atlantic salmon B cells to respond to TLR ligands was shown by demonstrating a constitutive expression of nucleic-acid sensing TLRs in magnetic sorted IgM+ cells. Of the two receptors recognizing CpG in teleosts, tlr9 was the dominating receptor with over ten-fold higher expression than tlr21. Upon CpG-stimulation, IgM secretion increased for head kidney (HK) and splenic IgM+ cells, while blood B cells were marginally affected. The results suggest that CpG directly affects salmon B cells to differentiate into antibody secreting cells (ASCs). IgM secretion was also detected in the non-treated controls, again with the highest levels in the HK derived population, signifying that persisting ASCs are present in this tissue. In all tissues, the IgM+ cells expressed high MHCII levels, suggesting antigen-presenting functions. Upon CpG-treatment the co-stimulatory molecules cd83 and cd40 were upregulated, while cd86 was down-regulated under the same conditions. Finally, ifna1 was upregulated upon CpG-stimulation in all tissues, while a restricted upregulation was evident for ifnb,...Continue Reading

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Citations

Jun 17, 2020·Frontiers in Immunology·Liting WuJun Li
Apr 12, 2019·Frontiers in Immunology·Rocío SimónCarolina Tafalla
Feb 26, 2019·Frontiers in Immunology·Chao-Yang LaiTsung-Hsien Chuang
Nov 13, 2020·Frontiers in Cell and Developmental Biology·Youngjin ParkViswanath Kiron

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Methods Mentioned

BETA
flow cytometry
magnetic
FACS
electrophoresis

Software Mentioned

FlowJo

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