Oct 20, 2009

Profiling carbohydrate-receptor interaction with recombinant innate immunity receptor-Fc fusion proteins

The Journal of Biological Chemistry
Tsui-Ling HsuChi-Huey Wong


The recognition of bacteria, viruses, fungi, and other microbes is controlled by host immune cells, which are equipped with many innate immunity receptors, such as Toll-like receptors, C-type lectin receptors, and immunoglobulin-like receptors. Our studies indicate that the immune modulating properties of many herbal drugs, for instance, the medicinal fungus Reishi (Ganoderma lucidum) and Cordyceps sinensis, could be attributed to their polysaccharide components. These polysaccharides specifically interact with and activate surface receptors involved in innate immunity. However, due to the complexity of polysaccharides and their various sources from medicinal fungi, quantitative analysis of medicinal polysaccharide extracts with regard to their functions represents a major challenge. To profile carbohydrate-immune receptor interactions, the extracellular domains of 17 receptors were cloned as Fc-fusion proteins, such that their interactions with immobilized polysaccharides could be probed in an enzyme-linked immunosorbent assay. The results show that several innate immune receptors, including Dectin-1, DC-SIGN, Langerin, Kupffer cell receptor, macrophage mannose receptor, TLR2, and TLR4, interact with the polysaccharide extract...Continue Reading

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Mentioned in this Paper

Kupffer cell receptor
Geranium lucidum
TLR4 gene
DC-specific ICAM-3 grabbing nonintegrin
Immunoglobulin Activity
Toll-Like Receptor 2
Cordyceps sinensis (fungus)

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