Profiling circular RNA in methamphetamine-treated primary cortical neurons identified novel circRNAs related to methamphetamine addiction

Neuroscience Letters
Junwei LiJinhao Sun

Abstract

Methamphetamine (METH) has been a worldwide health threat for years. Recent studies have reported that circular RNA (circRNA) are highly abundant and dynamically expressed in brain. However, connections between circRNA and METH-induced neurotoxicity remains indefinite. In the present study, primary cortical neurons were treated with METH in vitro. We profiled circRNA via high-throughput RNA sequencing and identified 2458 circRNAs. Bioinformatics analysis was performed to predict potential functions of these circRNAs which revealed several relevant pathways including 'morphine addiction' that may contribute to the pathogenesis of neuronal damage by METH. Especially, a METH-addicted mouse model was established with conditional place preference paradigm for validation of screened circRNAs. At last, we established co-expression networks of circRNAs with miRNAs and mRNAs to exhibit potential association among them. In conclusion, we firstly unveiled a role of circRNAs in METH-induced neuronal damage and METH addiction.

Citations

Apr 30, 2020·International Journal of Molecular Sciences·Erteng JiaQinyu Ge
May 18, 2019·Journal of Neurochemistry·Shanshan LuJie Yan
Dec 19, 2020·Wiley Interdisciplinary Reviews. RNA·Austin M GowenSowmya V Yelamanchili
Nov 24, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yueting Shao, Yiguo Jiang
Apr 26, 2021·Molecular and Cellular Biochemistry·Ying-Jian GuYun Wang
Jun 12, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Hailei YuChunling Ma
Aug 12, 2021·Journal of Molecular Neuroscience : MN·Junwei LiPeng Zhou

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