Profiling Early Humoral Response to Diagnose Novel Coronavirus Disease (COVID-19).

Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America
Li GuoJianwei Wang

Abstract

The emergence of coronavirus disease 2019 (COVID-19) is a major healthcare threat. The current method of detection involves a quantitative polymerase chain reaction (qPCR)-based technique, which identifies the viral nucleic acids when present in sufficient quantity. False-negative results can be achieved and failure to quarantine the infected patient would be a major setback in containing the viral transmission. We aim to describe the time kinetics of various antibodies produced against the 2019 novel coronavirus (SARS-CoV-2) and evaluate the potential of antibody testing to diagnose COVID-19. The host humoral response against SARS-CoV-2, including IgA, IgM, and IgG response, was examined by using an ELISA-based assay on the recombinant viral nucleocapsid protein. 208 plasma samples were collected from 82 confirmed and 58 probable cases (qPCR negative but with typical manifestation). The diagnostic value of IgM was evaluated in this cohort. The median duration of IgM and IgA antibody detection was 5 (IQR, 3-6) days, while IgG was detected 14 (IQR, 10-18) days after symptom onset, with a positive rate of 85.4%, 92.7%, and 77.9%, respectively. In confirmed and probable cases, the positive rates of IgM antibodies were 75.6% and 93...Continue Reading

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Methods Mentioned

BETA
PCR
bronchoalveolar lavage
electrophoresis
Infrared Imaging
enzyme-linked immunosorbent assay
ELISA
ELISAs
Assay

Software Mentioned

Fit Spline
MEGA
SPSS
ClustalW
GISAISD
Graphpad

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