Profiling monoterpenol glycoconjugation in Vitis vinifera L. cv. Muscat of Alexandria using a novel putative compound database approach, high resolution mass spectrometry and collision induced dissociation fragmentation analysis

Analytica Chimica Acta
Anna K HjelmelandSusan E Ebeler

Abstract

In this work we present a novel approach for the identification of plant metabolites using ultrahigh performance liquid chromatography coupled to accurate mass time-of-flight mass spectrometry. The workflow involves developing an in-house compound database consisting of exact masses of previously identified as well as putative compounds. The database is used to screen accurate mass spectrometry (MS) data to identify possible compound matches. Subsequent tandem MS data is acquired for possible matches and used for structural elucidation. The methodology is applied to profile monoterpene glycosides in Vitis vinifera cv. Muscat of Alexandria grape berries over three developmental stages. Monoterpenes are a subclass of terpenes, the largest class of plant secondary metabolites, and are found in two major forms in the plant, "bound" to one or more sugar moieties or "free" of said sugar moieties. In the free form, monoterpenes are noted for their fragrance and play important roles in plant defense and as attractants for pollinators. However, glycoconjugation renders these compounds odorless, and it is this form that the plant uses for monoterpene storage. In order to gain insight into monoterpene biochemistry and their fate in the pl...Continue Reading

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Citations

Feb 2, 2017·Journal of Food Science·Jibin LiuYong-Sheng Tao
Jan 4, 2018·Journal of the Science of Food and Agriculture·Marco SollazzoAndrea Bellincontro
Sep 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Silvia CarlinFulvio Mattivi
Dec 11, 2019·Biomolecules·Vicente Ferreira, Ricardo Lopez
Feb 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria TufarielloFrancesco Grieco
Mar 5, 2020·Journal of Agricultural and Food Chemistry·Andrew J CaffreySusan E Ebeler
Aug 30, 2017·Journal of Agricultural and Food Chemistry·Matthew NoesthedenWesley F Zandberg
Jul 3, 2021·Journal of Agricultural and Food Chemistry·Andrew J CaffreySusan E Ebeler

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