Profiling of measles-specific humoral immunity in individuals following two doses of MMR vaccine using proteome microarrays

Viruses
Iana H HaralambievaGregory A Poland

Abstract

Comprehensive evaluation of measles-specific humoral immunity after vaccination is important for determining new and/or additional correlates of vaccine immunogenicity and efficacy. We used a novel proteome microarray technology and statistical modeling to identify factors and models associated with measles-specific functional protective immunity in 150 measles vaccine recipients representing the extremes of neutralizing antibody response after two vaccine doses. Our findings demonstrate a high seroprevalence of antibodies directed to the measles virus (MV) phosphoprotein (P), nucleoprotein (N), as well as antibodies to the large polymerase (L) protein (fragment 1234 to 1900 AA). Antibodies to these proteins, in addition to anti-F antibodies (and, to a lesser extent, anti-H antibodies), were correlated with neutralizing antibody titer and/or were associated with and predictive of neutralizing antibody response. Our results identify antibodies to specific measles virus proteins and statistical models for monitoring and assessment of measles-specific functional protective immunity in vaccinated individuals.

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Citations

May 20, 2016·Medical Microbiology and Immunology·Eugenia Corrales-AguilarHartmut Hengel
Dec 27, 2018·Expert Review of Vaccines·Iana H HaralambievaGregory A Poland
Mar 19, 2021·Journal of Clinical Microbiology·Melissa M CoughlinBettina Bankamp

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Datasets Mentioned

BETA
AF266288

Methods Mentioned

BETA
proteomic profiling
chips
PCR
Protein Array
chip
Assay
ELISA

Software Mentioned

GenePix ® Pro 7 Microarray Acquisition Analysis
ImmunoSpot ®
Image
Pro Plus

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