Profiling of microRNAs involved in retinal degeneration caused by selective Müller cell ablation

PloS One
Sook Hyun ChungWeiyong Shen

Abstract

Dysfunction of Müller cells has been implicated in the pathogenesis of several retinal diseases. In order to understand the potential contribution of Müller cells to retinal disease better, we have developed a transgenic model in which foci of Müller cell ablation can be selectively induced. MicroRNAs (miRNAs), small non-coding RNAs that are involved in post-transcriptional modulation, have critical functions in various biological processes. The aim of this study was to profile differential expression of miRNAs and to examine changes in their target genes 2 weeks after Müller cell ablation. We identified 20 miRNAs using the miScript HC PCR array. Data analysis using two target gene prediction databases (TargetScan and mirTarBase) revealed 78 overlapping target genes. DAVID and KEGG pathway analysis suggested that the target genes were generally involved in cell apoptosis, p53, neurotrophin, calcium, chemokine and Jak-STAT signalling pathways. Changes in seven target genes including Cyclin D2, Caspase 9, insulin-like growth factor 1, IL-1 receptor-associated kinase (IRAK), calmodulin (CALM) and Janus kinase 2 (Jak2), were validated with qRT-PCR and western blots. The cellular localisation of cleaved-caspase 9, Cyclin D2, Jak2 an...Continue Reading

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Citations

Aug 17, 2016·Scientific Reports·Arpad PalfiG Jane Farrar
Jun 11, 2020·Clinical & Experimental Ophthalmology·Lan-Fang SunZi-Bing Jin
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Methods Mentioned

BETA
transgenic
Electrophoresis
PCR
confocal microscopy
acetylation
Assay

Software Mentioned

mirTarBAse
GeneTool
Relative Expression Software Tool ( REST )
KEGG
DAVID
TargetScan

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