Profiling of N- and O-glycopeptides of erythropoietin by capillary zwitterionic type of hydrophilic interaction chromatography/electrospray ionization mass spectrometry

Journal of Separation Science
Yasuhiro TakegawaShin-Ichiro Nishimura

Abstract

Capillary zwitterionic-type hydrophilic interaction chromatography (ZIC-HILIC)/ESI-MS has been applied to the Glu-C digest of recombinant human erythropoietin (rhEPO) expressed in Chinese hamster ovary (CHO) cells. N-Glycopeptides (105) and O-glycopeptides (8) were detected in a single run of the capillary ZIC-HILIC/ESI-MS analysis. Among them, N-acetyl-neuraminic acids (Neu5Ac) of N- and O-glycans were partially acetylated and some were replaced with N-glycoyl-neuraminic acid (Neu5Gc). Their retentions in the ZIC-HILIC separation can be explained to some extent with the degree of acetylation and N-glycoylation, both of which influence the hydrophilicity/hydrophobicity of the N- and O-glycan moieties of glycopeptides.

References

Jun 26, 2001·Glycoconjugate Journal·A Kobata
Feb 14, 2002·Chemical Reviews·Yehia Mechref, Milos V Novotny
Mar 28, 1996·Chemical Reviews·Raymond A. Dwek
Sep 10, 2002·Biologicals : Journal of the International Association of Biological Standardization·Miyako OhtaTakao Hayakawa
Jan 9, 2004·Analytical Chemistry·Hyun Joo AnCarlito B Lebrilla
Apr 30, 2005·Analytical Chemistry·Ruiqing Qiu, Fred E Regnier
Oct 20, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Manfred WuhrerCornelis H Hokke
Dec 13, 2006·Journal of Separation Science·Yasuhiro TakegawaShin-Ichiro Nishimura
Dec 30, 2006·Analytical Chemistry·Caterina TemporiniGabriella Massolini

❮ Previous
Next ❯

Citations

Nov 30, 2011·Bioanalysis·Serenus HuaHyun Joo An
Feb 8, 2013·Molecular & Cellular Proteomics : MCP·Heather Desaire
Mar 9, 2011·Journal of Pharmaceutical and Biomedical Analysis·Keita Yamada, Kazuaki Kakehi
Jul 20, 2016·Journal of Chromatography. a·Yahui PengXinmiao Liang
Aug 25, 2009·Current Opinion in Chemical Biology·Hyun Joo AnCarlito B Lebrilla
Jan 18, 2011·Proteomics·Martin Pabst, Friedrich Altmann
Mar 12, 2010·Journal of Separation Science·Gerhild ZaunerManfred Wuhrer
Sep 22, 2012·Journal of Separation Science·Sara OngayRainer Bischoff
Dec 18, 2013·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Shigeo Suzuki
Apr 25, 2020·Mass Spectrometry Reviews·David J Harvey
Nov 26, 2010·Therapeutic Drug Monitoring·Christina TsitsimpikouAristides Tsatsakis
May 4, 2016·Expert Review of Proteomics·Li CaoSi Wu
May 29, 2018·Analytical and Bioanalytical Chemistry·Petr KozlikMiloslav Sanda
Sep 23, 2008·Analytical Biochemistry·Esther LlopJosé A Pascual

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.