Profiling of phospholipids in lipoproteins by multiplexed hollow fiber flow field-flow fractionation and nanoflow liquid chromatography-tandem mass spectrometry

Journal of Chromatography. a
Ju Yong LeeMyeong Hee Moon

Abstract

This study describes a coupled analytical method to carry out the systematic profiling of phospholipids (PLs) in high-density lipoproteins (HDL) and low-density lipoproteins (LDL) from human blood plasma. HDL and LDL of healthy human plasma samples were separated by size and collected on a semi-preparative scale using multiplexed hollow fiber flow field-flow fractionation (MxHF5). Phospholipid mixtures contained in the resulting HDL and LDL fractions were analyzed by shotgun nanoflow liquid chromatography-tandem mass spectrometry (nLC-ESI-MS-MS). We utilized a dual scan method for the separation and simultaneous characterization of complicated PL mixtures by nLC-ESI-MS-MS, such that phosphatidylcholine (PC) and phosphatidylethanolamine (PE) molecules were detected in positive ion mode in a first LC run. In a second LC run, phosphatidylinositol (PI), phosphatidylglycerol (PG), and phosphatidic acid (PA) were detected in negative ion mode. In this study, a total of 56 PLs from HDL and 52 PLs from LDL particles were characterized by their molecular structures from data dependent collision-induced dissociation (CID) experiments, and their relative abundances were compared.

References

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Oct 29, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ilyong ParkMyeong Hee Moon
Mar 31, 2004·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Marcia M WrightVanina Zaremberg
Jul 1, 2005·Analytical Chemistry·Dukjin Kang, Myeong Hee Moon
Jul 2, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ryo TaguchiTakao Shimizu
Dec 2, 2005·Journal of Separation Science·Ilyong ParkMyeong Hee Moon
Feb 14, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dae Young BangMyeong Hee Moon
Nov 6, 2007·Progress in Lipid Research·Claude Wolf, Peter J Quinn
Nov 24, 2007·Clinical Chemistry·Diana Cristina RambaldiChristoph Johann
Aug 18, 2009·Journal of Chromatography. a·Ju Yong LeeMyeong Hee Moon

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Citations

Nov 4, 2010·Analytical and Bioanalytical Chemistry·Diana Cristina RambaldiAndrea Zattoni
Jul 17, 2010·American Journal of Therapeutics·Sreekanth Racherla, Rohit Arora
Nov 8, 2015·Pharmacology & Therapeutics·Maryam DarabiAnatol Kontush
Nov 1, 2015·Clinica Chimica Acta; International Journal of Clinical Chemistry·Sarika AroraReetika Saini
Oct 2, 2013·Journal of Clinical Lipidology·Peter P TothDaniel J Rader
Apr 2, 2013·Journal of Lipid Research·Anatol KontushM John Chapman
Nov 25, 2018·Analytical and Bioanalytical Chemistry·Carmen R M BriaS Kim Ratanathanawongs Williams
Apr 13, 2017·Journal of Chromatography. a·Carmen R M BriaS Kim Ratanathanawongs Williams
Nov 26, 2010·Analytical Chemistry·S Kim Ratanathanawongs WilliamsAkram A Ashames

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