Profiling of RNA degradation for estimation of post mortem [corrected] interval

PloS One
Fernanda Sampaio-SilvaRicardo Silvestre

Abstract

An estimation of the post mortem interval (PMI) is frequently touted as the Holy Grail of forensic pathology. During the first hours after death, PMI estimation is dependent on the rate of physical observable modifications including algor, rigor and livor mortis. However, these assessment methods are still largely unreliable and inaccurate. Alternatively, RNA has been put forward as a valuable tool in forensic pathology, namely to identify body fluids, estimate the age of biological stains and to study the mechanism of death. Nevertheless, the attempts to find correlation between RNA degradation and PMI have been unsuccessful. The aim of this study was to characterize the RNA degradation in different post mortem tissues in order to develop a mathematical model that can be used as coadjuvant method for a more accurate PMI determination. For this purpose, we performed an eleven-hour kinetic analysis of total extracted RNA from murine's visceral and muscle tissues. The degradation profile of total RNA and the expression levels of several reference genes were analyzed by quantitative real-time PCR. A quantitative analysis of normalized transcript levels on the former tissues allowed the identification of four quadriceps muscle gene...Continue Reading

References

Dec 16, 1994·Forensic Science International : Synergy·Burkhard Madea
Jan 5, 2000·Journal of Biotechnology·O ThellinE Heinen
Nov 16, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Jacques B de KokPaul N Span
Dec 21, 2004·Forensic Science International : Synergy·Stacey AndersonClifton P Bishop
Feb 22, 2005·BMC Molecular Biology·Xiaozhu ZhangAndrew J Sandford
Apr 9, 2005·Genes and Immunity·Jim HuggettA Zumla
Jun 1, 2005·Brain Research. Molecular Brain Research·Vibeke Sørensen CattsLouise Helen Lutze-Mann
Jun 9, 2005·Forensic Science International : Synergy·Burkhard Madea
Jun 9, 2005·Forensic Science International : Synergy·Jane Juusola, Jack Ballantyne
Feb 14, 2006·Molecular Aspects of Medicine·Simone Fleige, Michael W Pfaffl
Jun 27, 2006·Forensic Science International : Synergy·Claus Henssge, Burkhard Madea
Oct 19, 2006·Brain Research·Ana D StanCarol A Tamminga
Nov 23, 2006·International Journal of Legal Medicine·Marielle HeinrichUlrike Schmidt
May 24, 2007·BMC Genomics·Kouame E KouadjoJonny St-Amand
Aug 19, 2007·BMC Molecular Biology·Ann-Britt NygardMerete Fredholm
Sep 11, 2007·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Lijiang LiuLiang Liu
Sep 18, 2007·Journal of Forensic Sciences·Jane Juusola, Jack Ballantyne
Sep 20, 2007·PloS One·Hendrik J M de JongeArja ter Elst
Mar 1, 2007·Forensic Science International. Genetics·M Bauer
Jun 1, 2007·Forensic Science International. Genetics·Marielle HeinrichUlrike Schmidt
Feb 14, 2009·Forensic Science International. Genetics·C HaasA Kratzer
Jan 19, 2010·Methods : a Companion to Methods in Enzymology·C BeckerMichael W Pfaffl
Mar 20, 2010·International Journal of Legal Medicine·Antje KoppelkammMarielle Heinrich
Jul 10, 2010·Toxicology Mechanisms and Methods·Ricardo Jorge Dinis-OliveiraTeresa Magalhães
Aug 3, 2010·Forensic Science International : Synergy·Cornelius Courts, Burkhard Madea
Aug 14, 2010·Forensic Science International : Synergy·Sara PartemiAntonio Oliva
Aug 21, 2010·Forensic Science International : Synergy·Marielle Vennemann, Antje Koppelkamm
Aug 21, 2010·Forensic Science International : Synergy·Marielle Vennemann, Antje Koppelkamm
May 18, 2011·International Journal of Legal Medicine·Antje KoppelkammMarielle Vennemann

Citations

Mar 20, 2014·Forensic Science International : Synergy·Tingyi SunLiang Liu
Aug 27, 2013·Archives of Medical Research·Teresa MagalhãesRicardo Jorge Dinis-Oliveira
Mar 5, 2016·Analytical and Bioanalytical Chemistry·Richard H KaszynskiYasuhiro Ueno
Apr 15, 2016·Biomolecular Detection and Quantification·Monika SidovaRadek Sindelka
Jul 9, 2014·Science & Justice : Journal of the Forensic Science Society·Wen-Can LiLong Chen
Nov 27, 2015·International Journal of Legal Medicine·Viktor S PoórKatalin Sipos
Sep 1, 2015·Forensic Science, Medicine, and Pathology·Gulnaz T JavanShivani Soni
Jan 28, 2015·Forensic Science International. Genetics·Melanie GrabmüllerCornelius Courts
Apr 22, 2015·The American Journal of Forensic Medicine and Pathology·Teddric WilliamsGulnaz T Javan
Sep 24, 2013·Forensic Science International : Synergy·Lucas González-HerreraEnrique Villanueva
Jan 3, 2015·Forensic Science International : Synergy·S SharmaDeepak Kaul
Dec 23, 2014·PloS One·Anita van der LindenJ Wolter Oosterhuis
Mar 23, 2017·Forensic Science International : Synergy·M Colby HunterPeter A Noble
Feb 15, 2018·Nature Communications·Pedro G FerreiraRoderic Guigó
Feb 5, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Teslin S SandstromJonathan B Angel
Feb 5, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Joshua J Vásquez, Peter W Hunt
Mar 10, 2015·Analytical and Bioanalytical Chemistry·Takako SatoKoichi Suzuki
Aug 20, 2014·International Journal of Legal Medicine·Barbara MuciacciaRossana Cecchi
Jun 5, 2016·Forensic Science, Medicine, and Pathology·Burkhard Madea
Sep 16, 2016·International Journal of Legal Medicine·M van den BergeT Sijen
Mar 14, 2019·International Journal of Legal Medicine·Kyoung-Min ChoiStefan Pittner
Apr 22, 2020·Scientific Reports·Gulnaz T JavanJack Ballantyne
Mar 5, 2019·Indian Journal of Hematology & Blood Transfusion : an Official Journal of Indian Society of Hematology and Blood Transfusion·A KumarA Singh
Jul 3, 2020·Metabolomics : Official Journal of the Metabolomic Society·Ekaterina A ZelentsovaYuri P Tsentalovich
May 10, 2019·Metabolomics : Official Journal of the Metabolomic Society·Emanuela LocciErnesto d'Aloja
Jul 19, 2019·International Journal of Legal Medicine·Salvatore ScrivanoLuciana Caenazzo
Jul 5, 2019·Forensic Science International. Genetics·Cornelius CourtsWalther Parson

Related Concepts

Body Fluids
Cessation of Life
Genes
Muscle
RNA
Evaluation
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Visceral
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