Profiling of testis-specific long noncoding RNAs in mice

BMC Genomics
Seong Hyeon HongChunghee Cho

Abstract

Spermatogenesis, which is the complex and highly regulated process of producing haploid spermatozoa, involves testis-specific transcripts. Recent studies have discovered that long noncoding RNAs (lncRNAs) are novel regulatory molecules that play important roles in various biological processes. However, there has been no report on the comprehensive identification of testis-specific lncRNAs in mice. We performed microarray analysis of transcripts from mouse brain, heart, kidney, liver and testis. We found that testis harbored the highest proportion of tissue-specific lncRNAs (11%; 1607 of 14,256). Testis also harbored the largest number of tissue-specific mRNAs among the examined tissues, but the proportion was lower than that of lncRNAs (7%; 1090 of 16,587). We categorized the testis-specific lncRNAs and found that a large portion corresponded to long intergenic ncRNAs (lincRNAs). Genomic analysis identified 250 protein-coding genes located near (≤ 10 kb) 194 of the loci encoding testis-specific lincRNAs. Gene ontology (GO) analysis showed that these protein-coding genes were enriched for transcriptional regulation-related terms. Analysis of male germ cell-related cell lines (F9, GC-1 and GC-2) revealed that some of the testis-s...Continue Reading

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Citations

Apr 23, 2020·Reproduction, Fertility, and Development·Yu ZhuFei Sun
Jul 3, 2019·International Journal of Endocrinology·Qingtao YangKusheng Wu
May 6, 2020·Veterinary World·Berlin Pandapotan PardedeIman Supriatna
Nov 1, 2020·Reproductive Biology and Endocrinology : RB&E·Meghali Joshi, Singh Rajender
Aug 23, 2019·Molecular Therapy. Nucleic Acids·Li Zhang, Handong Wang
Mar 23, 2021·Frontiers in Cell and Developmental Biology·Adriana GeisingerRicardo Benavente
May 8, 2021·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Shaoyu GuoMingyou Li
Jun 22, 2021·Neurochemistry International·Mengqi ZhangKe-Jie Yin
Aug 28, 2021·Life·Marco BarchiPaola Grimaldi

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Datasets Mentioned

BETA
AK018904
GSE105024

Methods Mentioned

BETA
PCR
electrophoresis
chip
Feature Extraction

Software Mentioned

BLAST
Primer
UCSC genome browser
Feature Extraction
Arraystar
BLASTN
GeneSpring GX
DAVID
Ensemble

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