Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines

Cellular and Molecular Life Sciences : CMLS
P WangJ Renes

Abstract

Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation was screened, and 41 proteins that responded to blocking of secretion by 20 degrees C treatment and/or brefeldin A treatment were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25 are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an actively remodeling extracellular matrix and several factors involved in growth regulation.

Citations

Jan 29, 2010·Cellular and Molecular Life Sciences : CMLS·Edwin C M Mariman, Ping Wang
Feb 19, 2009·International Journal of Obesity : Journal of the International Association for the Study of Obesity·D M MutchK Clément
Sep 21, 2005·Nature Neuroscience·Josana RodriguezPaola Bovolenta
Jan 5, 2005·Nature Reviews. Immunology·Frank J T Staal, Hans C Clevers
Dec 5, 2006·Oncogene·A J Mikels, R Nusse
Sep 29, 2011·Molecular & Cellular Proteomics : MCP·Stefan LehrJuergen Eckel
Mar 30, 2012·Biomedical Materials·D A Young, K L Christman
Mar 17, 2007·Arthritis Research & Therapy·Tracey WeilerJohn A Wilkins
Oct 6, 2014·Nature Materials·Young-Wook WonYong-Hee Kim
Dec 20, 2012·Journal of Pregnancy·Ousseynou SarrTimothy R H Regnault
Mar 4, 2014·Cell Death and Differentiation·H ChoiJ-W Kim
Mar 4, 2015·International Journal of Molecular Sciences·Eun Young KimKwang-Hee Bae
Jan 26, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Anke Van SummerenEdwin C M Mariman
Mar 29, 2011·Journal of Proteomics·Arturo Roca-RivadaMaría Pardo
Oct 6, 2009·Molecular and Cellular Endocrinology·Chin Fen TeoLance Wells
Sep 9, 2008·Endocrinology and Metabolism Clinics of North America·Nils HalbergPhilipp E Scherer
Jun 28, 2015·Biochemical and Biophysical Research Communications·Rajib Mukherjee, Jong Won Yun
Jun 23, 2009·Biotechnology and Bioengineering·John F Woolley, Mohamed Al-Rubeai
Feb 1, 2009·Proteomics. Clinical Applications·Sílvia Barceló-Batllori, Ramon Gomis
Aug 9, 2006·The Journal of Clinical Hypertension·Thomas D Giles
Jul 11, 2008·Genes, Chromosomes & Cancer·Marlena S FejzoDennis J Slamon
Jan 4, 2012·Proteomics. Clinical Applications·Stefan LehrHenrike Sell
Dec 4, 2010·International Journal of Cancer. Journal International Du Cancer·Hajime SunagozakaShuichi Kaneko
Sep 9, 2015·Obesity·Lihong ZhangYun Bai
Apr 30, 2015·International Journal of Obesity : Journal of the International Association for the Study of Obesity·R MukherjeeJ W Yun
Dec 3, 2014·Biochimica Et Biophysica Acta·Michael Bukrinsky
Jun 11, 2016·Metabolism: Clinical and Experimental·Emanuel FrykPer-Anders Jansson
Jan 1, 2012·Adipocyte·David Brockman, Xiaoli Chen
Oct 12, 2010·Acta Biomaterialia·D Adam YoungKaren L Christman
Nov 28, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Catherine J DenisAnne-Marie Lambeir
Jul 26, 2008·Molecular Neurodegeneration·Nibaldo C Inestrosa, Enrique M Toledo
Jul 19, 2013·Mediators of Inflammation·Silja Raschke, Jürgen Eckel
Jan 13, 2015·Nature Medicine·Mortimer Korf-KlingebielKai C Wollert

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.