Profiling polar and semipolar plant metabolites throughout extraction processes using a combined solution-state and high-resolution magic angle spinning NMR approach

Analytical Chemistry
Yasuyo SekiyamaJun Kikuchi

Abstract

In metabolomic analyses, care should be exercised as to which metabolites are extracted from the sample and which remain in the residue; the remaining metabolites are typically discarded following the extraction process. In this study, nuclear magnetic resonance (NMR)-based metabolomics was used to visualize plant metabolite profiles throughout a series of repeated extraction processes. Metabolites remaining in the extraction residues of (13)C-labeled Arabidopsis thaliana were recovered by repeated extraction using methanol-d(4) and deuterium oxide. The soluble extracts and residual pellets from each step of the extraction process were analyzed by both solution-state and high-resolution magic angle spinning NMR. Metabolic profiling based on chemical shifts in two-dimensional (1)H-(13)C heteronuclear single-quantum coherence spectra allowed the elucidation of both structural and chemical properties. In addition to the metabolite profile, there appears to be a relationship between metabolite structure and behavior throughout the repeated extraction process. These approaches suggest that metabolites are not always extracted in a single step and that the distribution of metabolites in an extraction scenario cannot be predicted sole...Continue Reading

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Citations

Feb 7, 2012·Plant Biotechnology Reports·Yozo Okazaki, Kazuki Saito
Aug 1, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Akira YamazawaJun Kikuchi
Oct 15, 2011·Journal of Pharmaceutical and Biomedical Analysis·Seung-Ok YangHyung-Kyoon Choi
Sep 4, 2012·Carbohydrate Polymers·Tetsuya MoriJun Kikuchi
Jan 22, 2014·Food Chemistry·Yasuhiro DateJun Kikuchi
Mar 5, 2014·Current Opinion in Biotechnology·Marcus Foston
Oct 12, 2012·Scientific Reports·Charlotte E NorrisRoderick E Wasylishen
Dec 21, 2017·PloS One·Misao ItougaHitoshi Sakakibara
Jan 28, 2021·International Journal of Molecular Sciences·Shunji YamadaJun Kikuchi
Feb 7, 2018·Progress in Nuclear Magnetic Resonance Spectroscopy·Jun KikuchiYasuhiro Date
May 15, 2015·Environmental Science & Technology·Takanori KomatsuJun Kikuchi

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