Profiling primary metabolites of tomato fruit with gas chromatography/mass spectrometry

Methods in Molecular Biology
Sonia OsorioAlisdair R Fernie

Abstract

Metabolite profiling is a rapidly expanding technology which aims to quantify the entire metabolome of biological samples. Gas Chromatography Mass Spectrometry (GC-MS) is one of the most widely used analytical tools for profiling highly complex mixtures of primary metabolites, such as organic and amino acids, sugars, sugars alcohols, phosphorylated intermediates, and lipophilic compounds. This chapter summarizes all of the preparatory steps for metabolite profiling of polar compounds by GC-MS in tomato fruit, from the sampling of plant material to the derivatization procedures required to render the metabolites volatile.

Citations

Mar 25, 2014·Journal of Experimental Botany·Adriana BastíasJosé A Casaretto
Apr 12, 2013·Plant Biology·A FlorianA R Fernie
Jan 1, 2014·The Plant Journal : for Cell and Molecular Biology·Luisa BermúdezFernando Carrari
Nov 29, 2014·Journal of Biosciences·Rahul Kumar, Ashima Khurana
Sep 19, 2019·Plant Biotechnology Journal·José G VallarinoSonia Osorio
Oct 10, 2019·The New Phytologist·Vikas DevkarSalma Balazadeh
Jan 28, 2020·The Plant Journal : for Cell and Molecular Biology·Sanu ShameerLee J Sweetlove
Feb 18, 2015·Plant, Cell & Environment·David B MedeirosWagner L Araújo
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Claudio Inostroza-BlancheteauAdriano Nunes-Nesi
Jan 11, 2021·Journal of the Science of Food and Agriculture·Yaoyao ZhaoYuquan Duan

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