Profiling the changes in signaling pathways in ascorbic acid/β-glycerophosphate-induced osteoblastic differentiation

Journal of Cellular Biochemistry
Antonio Hernandes Chaves NetoCarmen Veríssima Ferreira

Abstract

Despite numerous reports on the ability of ascorbic acid and β-glycerophosphate (AA/β-GP) to induce osteoblast differentiation, little is known about the molecular mechanisms involved in this phenomenon. In this work, we used a peptide array containing specific consensus sequences (potential substrates) for protein kinases and traditional biochemical techniques to examine the signaling pathways modulated during AA/β-GP-induced osteoblast differentiation. The kinomic profile obtained after 7 days of treatment with AA/β-GP identified 18 kinase substrates with significantly enhanced or reduced phosphorylation. Peptide substrates for Akt, PI3K, PKC, BCR, ABL, PRKG1, PAK1, PAK2, ERK1, ERBB2, and SYK showed a considerable reduction in phosphorylation, whereas enhanced phosphorylation was observed in substrates for CHKB, CHKA, PKA, FAK, ATM, PKA, and VEGFR-1. These findings confirm the potential usefulness of peptide microarrays for identifying kinases known to be involved in bone development in vivo and in vitro and show that this technique can be used to investigate kinases whose function in osteoblastic differentiation is poorly understood.

References

Feb 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R T Franceschi, B S Iyer
Jun 1, 1992·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·L D QuarlesR J Wenstrup
Oct 1, 1992·Calcified Tissue International·C H ChungI M Shapiro
Mar 1, 1992·Nutrition Reviews·R T Franceschi
Dec 1, 1995·The Journal of Biological Chemistry·A C Newton
Oct 13, 1995·The Journal of Biological Chemistry·S ZhangG M Bokoch
Jun 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R T FranceschiY Cui
Apr 27, 2000·Current Opinion in Immunology·T Kurosaki
Aug 4, 2001·Biochemical and Biophysical Research Communications·R InatomeH Yamamura
Mar 22, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kun JiangJulie Y Djeu
May 15, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Roger RezzonicoHeidy Schmid-Antomarchi
May 16, 2003·Nature·Shun-ichi Harada, Gideon A Rodan
Sep 10, 2004·The Journal of Biological Chemistry·Sander H DiksMaikel P Peppelenbosch
Mar 22, 2005·Archives of Oral Biology·Francesco CarinciAdriano Piattelli
May 4, 2006·The Journal of Clinical Investigation·Venkatesh KrishnanOrmond A Macdougald
Jun 7, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Nalini H KulkarniYanfei L Ma
Jan 26, 2007·International Journal of Oral and Maxillofacial Surgery·J-H ByunJ-H Lee
Jun 9, 2007·Development, Growth & Differentiation·Melanie C FisherCaroline N Dealy
Nov 29, 2007·Blood·Stephen FitterAndrew C W Zannettino
Feb 19, 2008·Cancer Research·Jurriaan B TuynmanDirk J Richel
Sep 11, 2008·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Keisuke KitaToru Nakano
Nov 8, 2008·Molecular and Cellular Biochemistry·Willian F ZambuzziCarmen V Ferreira
Dec 18, 2008·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Willian F ZambuzziCarmen V Ferreira
Feb 3, 2009·Journal of Bone and Mineral Metabolism·Chikahisa HiguchiKazuyuki Itoh
Feb 17, 2009·Biochemical and Biophysical Research Communications·Chafik GhayorFranz E Weber
Jun 17, 2009·Journal of Orthopaedic Surgery and Research·John Street, Brian Lenehan

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Citations

Jan 1, 2012·Scientifica·Maikel P Peppelenbosch
Nov 19, 2013·Clinical & Experimental Metastasis·Sandy R LarsonColm Morrissey
Feb 26, 2011·Biotechnology and Bioengineering·Willian F ZambuzziJosé M Granjeiro
Apr 29, 2016·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Luis Cezar Farias de OliveiraSandra Helena Penha Oliveira
Aug 5, 2019·Journal of Ethnopharmacology·Victor Gustavo Balera BritoSandra Helena Penha Oliveira

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