May 25, 2011

Profiling the venom gland transcriptomes of Costa Rican snakes by 454 pyrosequencing

BMC Genomics
Jordi DurbanJuan J Calvete

Abstract

A long term research goal of venomics, of applied importance for improving current antivenom therapy, but also for drug discovery, is to understand the pharmacological potential of venoms. Individually or combined, proteomic and transcriptomic studies have demonstrated their feasibility to explore in depth the molecular diversity of venoms. In the absence of genome sequence, transcriptomes represent also valuable searchable databases for proteomic projects. The venom gland transcriptomes of 8 Costa Rican taxa from 5 genera (Crotalus, Bothrops, Atropoides, Cerrophidion, and Bothriechis) of pitvipers were investigated using high-throughput 454 pyrosequencing. 100,394 out of 330,010 masked reads produced significant hits in the available databases. 5.165,220 nucleotides (8.27%) were masked by RepeatMasker, the vast majority of which corresponding to class I (retroelements) and class II (DNA transposons) mobile elements. BLAST hits included 79,991 matches to entries of the taxonomic suborder Serpentes, of which 62,433 displayed similarity to documented venom proteins. Strong discrepancies between the transcriptome-computed and the proteome-gathered toxin compositions were obvious at first sight. Although the reasons underlaying thi...Continue Reading

  • References114
  • Citations54

References

  • References114
  • Citations54

Citations

Mentioned in this Paper

PLA2G1B gene
Choeroniscus godmani
Short Tandem Repeat
Atropoides
Brycinus lateralis
QPCT gene
PLA2G2A gene
PLA2G2A protein, human
Snake Venoms
Crotalus durissus

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