Progenitor cells in auricular cartilage demonstrate cartilage-forming capacity in 3D hydrogel culture

European Cells & Materials
Iris A OttoJ Malda

Abstract

Paramount for the generation of auricular structures of clinically-relevant size is the acquisition of a large number of cells maintaining an elastic cartilage phenotype, which is the key in producing a tissue capable of withstanding forces subjected to the auricle. Current regenerative medicine strategies utilize chondrocytes from various locations or mesenchymal stromal cells (MSCs). However, the quality of neo-tissues resulting from these cell types is inadequate due to inefficient chondrogenic differentiation and endochondral ossification, respectively. Recently, a subpopulation of stem/progenitor cells has been identified within the auricular cartilage tissue, with similarities to MSCs in terms of proliferative capacity and cell surface biomarkers, but their potential for tissue engineering has not yet been explored. This study compared the in vitro cartilage-forming ability of equine auricular cartilage progenitor cells (AuCPCs), bone marrow-derived MSCs and auricular chondrocytes in gelatin methacryloyl (gelMA)-based hydrogels over a period of 56 d, by assessing their ability to undergo chondrogenic differentiation. Neocartilage formation was assessed through gene expression profiling, compression testing, biochemical co...Continue Reading

Citations

Aug 31, 2020·Laryngoscope Investigative Otolaryngology·Weston L NiermeyerTendy Chiang
Jul 28, 2020·Medical Engineering & Physics·Gareth TurnbullWenmiao Shu
Jun 3, 2021·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Sarah HumphriesRahul Kanegaonkar
Oct 8, 2021·Tissue Engineering and Regenerative Medicine·Guanhuier WangZhenmin Zhao

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