Progenitor Renin Lineage Cells are not involved in the regeneration of glomerular endothelial cells during experimental renal thrombotic microangiopathy

PloS One
Leo RuhnkeChristian P M Hugo

Abstract

Endothelial cells (EC) frequently undergo primary or secondary injury during kidney disease such as thrombotic microangiopathy or glomerulonephritis. Renin Lineage Cells (RLCs) serve as a progenitor cell niche after glomerular damage in the adult kidney. However, it is not clear whether RLCs also contribute to endothelial replenishment in the glomerulus following endothelial injury. Therefore, we investigated the role of RLCs as a potential progenitor niche for glomerular endothelial regeneration. We used an inducible tet-on triple-transgenic reporter strain mRen-rtTAm2/LC1/LacZ to pulse-label the renin-producing RLCs in adult mice. Unilateral kidney EC damage (EC model) was induced by renal artery perfusion with concanavalin/anti-concanavalin. In this model glomerular EC injury and depletion developed within 1 day while regeneration occurred after 7 days. LacZ-labelled RLCs were restricted to the juxtaglomerular compartment of the afferent arterioles at baseline conditions. In contrast, during the regenerative phase of the EC model (day 7) a subset of LacZ-tagged RLCs migrated to the glomerular tuft. Intraglomerular RLCs did not express renin anymore and did not stain for glomerular endothelial or podocyte cell markers, but fo...Continue Reading

References

Nov 30, 2007·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Bernd HohensteinChristian P M Hugo
Apr 16, 2010·Physiological Reviews·Hayo CastropCharlotte Wagner
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Jul 5, 2013·PloS One·Sunder Sims-LucasCarlton M Bates
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Jun 7, 2014·Journal of the American Society of Nephrology : JASN·Charlotte StarkeChristian P M Hugo
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Aug 5, 2017·Journal of the American Society of Nephrology : JASN·Peter LachmannVladimir T Todorov

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Citations

Apr 12, 2020·Therapeutic Apheresis and Dialysis : Official Peer-reviewed Journal of the International Society for Apheresis, the Japanese Society for Apheresis, the Japanese Society for Dialysis Therapy·Paul S Malchesky, Tadao Akizawa
Aug 10, 2019·Pflügers Archiv : European journal of physiology·Armin Kurtz
Aug 8, 2019·Pflügers Archiv : European journal of physiology·Anne SteglichVladimir T Todorov
Apr 2, 2021·Circulation Research·Maria Luisa S Sequeira-Lopez, R Ariel Gomez

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Methods Mentioned

BETA
confocal microscopy

Software Mentioned

Imaris
Graph Pad

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