Progenitor/Stem Cell Markers in Brain Adjacent to Glioblastoma: GD3 Ganglioside and NG2 Proteoglycan Expression

Journal of Neuropathology and Experimental Neurology
Gina LamaGigliola Sica

Abstract

Characterization of tissue surrounding glioblastoma (GBM) is a focus for translational research because tumor recurrence invariably occurs in this area. We investigated the expression of the progenitor/stem cell markers GD3 ganglioside and NG2 proteoglycan in GBM, peritumor tissue (brain adjacent to tumor, BAT) and cancer stem-like cells (CSCs) isolated from GBM (GCSCs) and BAT (PCSCs). GD3 and NG2 immunohistochemistry was performed in paired GBM and BAT specimens from 40 patients. Double-immunofluorescence was carried out to characterize NG2-positive cells of vessel walls. GD3 and NG2 expression was investigated in GCSCs and PCSCs whose tumorigenicity was also evaluated in Scid/bg mice. GD3 and NG2 expression was higher in tumor tissue than in BAT. NG2 decreased as the distance from tumor margin increased, regardless of the tumor cell presence, whereas GD3 correlated with neoplastic infiltration. In BAT, NG2 was coexpressed with a-smooth muscle actin (a-SMA) in pericytes and with nestin in the endothelium. Higher levels of NG2 mRNA and protein were found in GCSCs while GD3 synthase was expressed at similar levels in the 2 CSC populations. PCSCs had lower tumorigenicity than GCSCs. These data suggest the possible involvement of...Continue Reading

References

Sep 1, 1980·Neurology·F H Hochberg, A Pruitt
Jun 24, 1996·Cancer Letters·S Koochekpour, G J Pilkington
Apr 1, 1996·Glia·J E Goldman, R Reynolds
Apr 30, 1998·Journal of the Neurological Sciences·A BakshiC Sarkar
Apr 14, 1999·Psychiatry and Clinical Neurosciences·K KawaiS Kuroda
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 10, 2003·Neuroscience Letters·Alessia MelaniFelicita Pedata
Nov 19, 2004·Nature·Sheila K SinghPeter B Dirks
Feb 22, 2005·Neoplasia : an International Journal for Oncology Research·Dominique B HoelzingerMichael E Berens
Mar 11, 2005·The New England Journal of Medicine·Roger StuppUNKNOWN National Cancer Institute of Canada Clinical Trials Group
Jan 18, 2006·Cancer Letters·Lionel M L Chow, Suzanne J Baker
Jun 24, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Marcela AssanahPeter Canoll
Dec 13, 2006·Advances in Experimental Medicine and Biology·Suzana CubillosLucimey Lima
Jun 16, 2007·Journal of Neuro-oncology·Tobias BirnbaumAndreas Straube
Nov 3, 2007·Genes & Development·Frank B FurnariWebster K Cavenee
Dec 7, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Annunziato MangiolaGigliola Sica
Mar 6, 2008·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Markus DehnhardtAndreas Bauer
Apr 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nikolaos A DallasLee M Ellis
Sep 6, 2008·Science·D Williams ParsonsKenneth W Kinzler
Mar 6, 2009·Cell Adhesion & Migration·William B Stallcup, Feng-Ju Huang
Sep 12, 2009·Experimental & Molecular Medicine·Ji-Ung JungYoung-Kug Choo
Jul 17, 2010·American Journal of Medical Genetics. Part a·Laura BernardiniBruno Dallapiccola
Aug 10, 2010·Annals of Neurology·Martin GlasBjörn Scheffler
May 7, 2011·Journal of Neuro-oncology·Tobias BirnbaumAndreas Straube
Mar 2, 2012·Glia·Nikki A CharlesHelmut Kettenmann
Mar 9, 2013·PloS One·Annunziato MangiolaCarmelo Anile
Jan 1, 2012·Cancers·Mihaela Lorger
Jan 7, 2014·Acta Biochimica Et Biophysica Sinica·Yuhan ZhaoWenwei Hu
May 3, 2014·American Journal of Physiology. Cell Physiology·Alexander BirbrairOsvaldo Delbono

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Citations

Feb 1, 2019·Journal of Cellular Physiology·Giacomo PozzoliCarlo Cenciarelli
Apr 17, 2019·Cancers·Alessio D'AlessioBianca Maria Scicchitano
Sep 15, 2018·Cancer Cell International·Bianca Maria ScicchitanoGigliola Sica
May 16, 2017·Laboratory Investigation; a Journal of Technical Methods and Pathology·Qi-Wen ZhaoXiu-Wu Bian
Aug 18, 2018·The Biochemical Journal·Anthony J Hayes, James Melrose
May 6, 2019·International Journal of Molecular Sciences·Cristiana AngelucciGigliola Sica
Feb 11, 2021·International Journal of Molecular Sciences·Cristiana AngelucciFortunata Iacopino
Nov 3, 2020·Frontiers in Immunology·Margarita BartishH Uri Saragovi
Mar 22, 2021·Fluids and Barriers of the CNS·Francesco GirolamoDaniela Virgintino
Jul 3, 2021·Brain Sciences·Filippo BiamonteAlessio D'Alessio

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