Progesterone and allopregnanolone reduce inflammatory cytokines after traumatic brain injury

Experimental Neurology
Jun HeDonald G Stein

Abstract

Following a traumatic brain injury (TBI), the excessive release of interleukin-1beta (IL-1beta) and tumor necrosis factor alpha (TNF-alpha) is a major cause of cerebral edema, which, in turn, can cause permanent neuronal loss and cognitive deficits in laboratory rats. This study examined the changes in expression of the proinflammatory cytokines IL-1beta and TNF-alpha after progesterone (8 mg/kg) or allopregnanolone (4 mg/kg) treatment in brain-injured rats at 3, 8, and 12 h and 6 days post-injury. Adult male rats received either bilateral prefrontal cortical contusion or sham surgery. The hormones were given intraperitoneally at 1 and 6 h, and continued once per day for up to 5 days. The gene expression of IL-1beta and TNF-alpha was measured by mRNA using real-time quantitative reverse transcripted polymerase chain reaction (RT-PCR). The protein concentrations of IL-1beta and TNF-alpha were measured using enzyme-linked immunosorbent assay (ELISA) to confirm the translation from mRNA to protein. The results indicated that progesterone and allopregnanolone reduce both IL-1beta and TNF-alpha at 3 h post-injury, when the expression of these cytokines peaks. At 8 and 12 h post-injury, IL-1beta and TNF-alpha gene expression in injur...Continue Reading

References

Apr 15, 1992·Annals of the New York Academy of Sciences·F Berkenbosch
Dec 1, 1989·The Journal of Clinical Endocrinology and Metabolism·M L PolanK Bottomly
Sep 1, 1987·JPEN. Journal of Parenteral and Enteral Nutrition·L OttC McClain
Jun 1, 1995·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·N J Rothwell, P J Strijbos
May 1, 1995·Brain Research. Molecular Brain Research·L FanT K McIntosh
Apr 1, 1995·Stroke; a Journal of Cerebral Circulation·Y YamasakiK Kogure
Jun 9, 1995·Science·H L KoenigE E Baulieu
Jan 1, 1994·British Journal of Neurosurgery·S A RossB J Rowlands
Aug 1, 1994·Journal of Neurotrauma·S W HoffmanD G Stein
Jan 1, 1996·Journal of Neuroimmunology·H E de VriesJ Kuiper
May 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·E ShohamiR Gallily
Sep 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·S A Loddick, N J Rothwell
Jan 1, 1996·Neuroscience and Biobehavioral Reviews·B ArvinG Z Feuerstein
Mar 1, 1996·Brain Research. Molecular Brain Research·L FanT K McIntosh
May 1, 1997·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H UnoM Sugita
Sep 1, 1996·Neuroimmunomodulation·E Di SantoP Ghezzi
May 1, 1997·Molecular and Chemical Neuropathology·R L RoofD G Stein
Aug 24, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·V TrembovlerE Shohami
Mar 1, 2000·Journal of Neuroimmunology·O TouzaniN Rothwell
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·S M AllanN J Rothwell
Jul 13, 2000·International Journal of Molecular Medicine·A L SalzmanC Szabo
Nov 7, 2000·Journal of Neuroimmunology·P D Drew, J A Chavis
Feb 13, 2001·Journal of Neuroimmunology·M Schwartz, G Moalem
Jun 5, 2013·Frontiers in Behavioral Neuroscience·Erica M JohnsonJames P Herman

❮ Previous
Next ❯

Citations

Apr 1, 2009·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Chao JiangYujin Hao
Mar 24, 2006·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Mathew B PottsLinda J Noble-Haeusslein
Apr 3, 2009·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Christine E MarxLawrence J Shampine
Feb 1, 2007·Journal of Neurotrauma·Annadora J Bruce-KellerStephen W Scheff
Mar 1, 2006·Journal of Neurotrauma·Daniel P DavisDavid B Hoyt
May 15, 2008·Journal of Neurotrauma·Lesley K GilmerStephen W Scheff
Jul 21, 2011·Journal of Neurotrauma·Gail D AndersonMichael R Hoane
Jan 31, 2013·Journal of Neurotrauma·J Regino Perez-PoloClaire E Hulsebosch
Sep 8, 2005·Epilepsy Currents·Helen E Scharfman
Nov 16, 2012·Journal of Psychopharmacology·William R KellerDeanna L Kelly
Jul 4, 2007·Journal of Neuroinflammation·Anthony J WilliamsFrank C Tortella
Apr 28, 2010·Journal of Brachial Plexus and Peripheral Nerve Injury·Paolo MilaniAlessandro Rossi
Sep 12, 2012·Journal of Molecular Endocrinology·Silvia GiattiBarbara Viviani
Mar 11, 2009·Frontiers in Bioscience (Landmark Edition)·Arnaud Nicot
Sep 10, 2013·International Journal of Molecular Sciences·Doodipala Samba Reddy, Ramkumar Kuruba
Dec 18, 2013·European Journal of Pharmacology·Pushpender KumarPyare Lal Sharma
Sep 10, 2013·Progress in Neurobiology·Paula J BruntonJonathan J Hirst
Oct 15, 2013·Progress in Neurobiology·Roberto Cosimo Melcangi, Gian Carlo Panzica
Aug 3, 2012·Journal of Toxicology and Environmental Health. Part a·Roger Lyrio dos SantosJones Bernardes Graceli
Jul 18, 2014·Psychopharmacology·Christine E MarxSiow Ann Chong
May 23, 2014·Psychopharmacology·Crystal Edler SchillerDavid R Rubinow
Jun 18, 2010·Canadian Journal of Physiology and Pharmacology·Nader ShahrokhiNouzar Nakhaee
Dec 28, 2010·Canadian Journal of Physiology and Pharmacology·Mohammad KhaksariGholamreza Asadikaram
Jul 27, 2007·Climacteric : the Journal of the International Menopause Society·S C Gilliver, G S Ashcroft
Nov 26, 2013·Brain Injury : [BI]·Helen W PhippsJacob W Vanlandingham

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