Progesterone and prostaglandin production by primate luteal cells collected at various stages of the luteal phase: modulation by calcium ionophore

Biology of Reproduction
B S Houmard, J S Ottobre

Abstract

Prostaglandins (PG) are produced by the corpus luteum (CL) of the rhesus monkey and may be involved in luteal regulation. Intracellular calcium has also been implicated as a mediator of luteolysis in domestic and laboratory species; however, its role in primate luteal function has not been investigated. The objectives of this study were to characterize temporal changes in basal and stimulated luteal PG production by CL of rhesus monkeys, and to examine the effects of calcium ionophore (CaI) on basal and gonadotropin-stimulated progesterone (P) production by the CL. CL were collected at various times after the estimated day of the luteinizing hormone (LH) surge: 5 days (early luteal phase, n = 4), 8-10 days (mid-luteal phase, n = 8), and 12-14 days (late luteal phase, n = 5). Dispersed luteal cells were incubated in the absence and presence of CaI, or with human chorionic gonadotropin (hCG) plus CaI at 37 degrees C for 8 h. PG and P concentrations in the medium were measured by radioimmunoassay. PGE2 and 6-keto-PGF1 alpha production decreased (p less than 0.05) from early luteal phase to mid-luteal phase and remained lower (p less than 0.05) during late luteal phase for all treatment groups. PGF2 alpha production decreased (p le...Continue Reading

Citations

Aug 1, 1994·Molecular and Cellular Endocrinology·B S HoumardJ S Ottobre
Feb 1, 1994·Molecular and Cellular Endocrinology·A E MichaelG E Webley
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Milo C Wiltbank, Joseph S Ottobre
Jan 1, 1993·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D L BooneP C Leung
Nov 1, 1994·Journal of Endocrinological Investigation·C NappiC Di Carlo
Apr 30, 1999·Physiological Reviews·J A McCrackenJ C Lamsa
Apr 1, 1994·Molecular and Cellular Endocrinology·J Olofsson, P C Leung

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