PMID: 6986169Jan 22, 1980Paper

Progesterone-binding components of chick oviduct: partial purification and characterization of a calcium-activated protease which hydrolyzes the progesterone receptor

Biochemistry
W V VedeckisB W O'Malley

Abstract

A calcium (Ca2+)-activated protease has been purified from laying hen oviducts. This enzyme can catalyze the limited proteolysis of the native chick oviduct progesterone receptor subunits, A and B, to smaller hormone-binding fragments. The protocol used has resulted in a 2000-fold purification of the enzyme in 40% yield from hen oviduct postmitochondrial supernatant fractions. This resulted in an active, purified protease preparation which can be used as an analytical tool for studying receptor protein structure. Characterization of the purified enzyme has shown that it is activated by Ca2+ (0.5-1 mM), has a molecular weight of 113 000, and has a sedimentation value of 6 S. No effect of calmodulin (Ca2+-dependent regulator) could be shown on the enzymatic activity of the protease. The enzyme has a Km of 1.04 x 10(-8) M for the receptor protein substrate. The protease is inactivated by sulfhydryl attacking reagents and thus can be classified as a sulfhydryl protease. This protease exhibits remarkable similarities to the "receptor transforming factor (RTF)", a Ca2+-activated protease which performs a limited proteolysis on the calf uterine estrogen receptor [Puca, G. A., Nola, E., Sica, V., & Bresciani, F. (1977) J. Biol. Chem. 2...Continue Reading

References

Feb 1, 1975·Biology of Reproduction·W T SchraderB W O'Malley
Jan 1, 1979·Journal of Steroid Biochemistry·W A CotyB W O'Malley
Mar 11, 1977·Annals of the New York Academy of Sciences·W T SchraderB W Malley
Oct 1, 1976·Journal of Steroid Biochemistry·P E HansenB W O'Malley
Jan 1, 1960·Annual Review of Biochemistry·B S HARTLEY

❮ Previous
Next ❯

Citations

Jun 1, 1983·Journal of Steroid Biochemistry·M KalimiA Ray
Jan 1, 1984·Journal of Steroid Biochemistry·P TuohimaaE E Baulieu
Dec 1, 1985·Journal of Steroid Biochemistry·W J Hendry, B J Danzo
Sep 1, 1986·Journal of Steroid Biochemistry·W J Hendry, B J Danzo
Mar 1, 1990·Journal of Steroid Biochemistry·J P CarboneR L Brent
Jan 1, 1984·Progress in Neurobiology·U J Zimmerman, W W Schlaepfer
Oct 1, 1983·Molecular and Cellular Endocrinology·K P Henrikson, H W Dickerman
Aug 1, 1984·Molecular and Cellular Endocrinology·J M Renoir, J Mester
Jul 26, 1985·Biochimica Et Biophysica Acta·F D UchimaH A Bern
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J H AldstadtP Johnson
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R W Evans, W W Leavitt
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·G A PucaA M Molinari
Nov 11, 1993·Nucleic Acids Research·F Watt, P L Molloy
Jun 1, 1981·European Journal of Biochemistry·H P Voorheis, B R Martin
Aug 24, 2001·Biological Chemistry·Y OzakiK Suzumori
Sep 14, 1981·Life Sciences·S Ishiura
Apr 1, 1986·Experimental Cell Research·J E Schollmeyer
Feb 15, 1988·Archives of Biochemistry and Biophysics·A Poland, E Glover
Nov 1, 1985·Archives of Biochemistry and Biophysics·M HatanakaT Murachi
Jun 30, 1987·Biochemical and Biophysical Research Communications·S PontremoliG Bianchi
Jul 1, 1982·Metabolism: Clinical and Experimental·W T Schrader
May 16, 1984·Biochemical and Biophysical Research Communications·U J Zimmerman, W W Schlaepfer
Oct 15, 1982·Biochemical and Biophysical Research Communications·M E Baker, W R Kimlinger
Jan 30, 1981·Biochemical and Biophysical Research Communications·T Hazato, A Murayama
Sep 16, 1982·Biochemical and Biophysical Research Communications·J E BishopA W Norman
Jun 15, 1982·Biochemical and Biophysical Research Communications·E MelloniS Pontremoli
Aug 1, 1985·Analytical Biochemistry·R Gopalakrishna, S H Barsky
May 14, 1986·Biochemical and Biophysical Research Communications·Y AdachiM Hatanaka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.