Progesterone from the cumulus cells is the sperm chemoattractant secreted by the rabbit oocyte cumulus complex.

PloS One
H A GuidobaldiLaura C Giojalas

Abstract

Sperm chemotaxis in mammals have been identified towards several female sources as follicular fluid (FF), oviduct fluid, and conditioned medium from the cumulus oophorus (CU) and the oocyte (O). Though several substances were confirmed as sperm chemoattractant, Progesterone (P) seems to be the best chemoattractant candidate, because: 1) spermatozoa express a cell surface P receptor, 2) capacitated spermatozoa are chemotactically attracted in vitro by gradients of low quantities of P; 3) the CU cells produce and secrete P after ovulation; 4) a gradient of P may be kept stable along the CU; and 5) the most probable site for sperm chemotaxis in vivo could be near and/or inside the CU. The aim of this study was to verify whether P is the sperm chemoattractant secreted by the rabbit oocyte-cumulus complex (OCC) in the rabbit, as a mammalian animal model. By means of videomicroscopy and computer image analysis we observed that only the CU are a stable source of sperm attractants. The CU produce and secrete P since the hormone was localized inside these cells by immunocytochemistry and in the conditioned medium by enzyme immunoassay. In addition, rabbit spermatozoa express a cell surface P receptor detected by western blot and localiz...Continue Reading

References

Oct 1, 1991·Molecular Reproduction and Development·C HansenB R Downey
Jun 4, 1971·Nature·P L PearsonP W Barlow
Jan 1, 1970·The Journal of Experimental Zoology·M J Harper
Jan 1, 1982·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·R M Hoar
May 1, 1996·Biology of Reproduction·K SabeurS Meizel
Mar 20, 1998·The Journal of Clinical Endocrinology and Metabolism·M LuconiE Baldi
Aug 6, 2000·Human Reproduction·A E CalogeroR D'Agata
Mar 22, 2003·Developmental Biology·Fei SunMichael Eisenbach
Jun 1, 1964·Journal of Reproduction and Fertility·P J BREDDERMANA M YASSEN
Nov 23, 2005·International Journal of Andrology·Chirag ShahChander Puri
Dec 21, 2005·Reproduction, Fertility, and Development·Jui-Te WuFeng-Pang Cheng
Apr 12, 2006·Nature Reviews. Molecular Cell Biology·Michael Eisenbach, Laura C Giojalas
Jun 21, 2006·Fertility and Sterility·María Eugenia TevesLaura Cecilia Giojalas
Dec 21, 2006·Animal Reproduction Science·Ying LiuThomas D Bunch
Mar 3, 2007·Nature Cell Biology·Stephen PublicoverChristopher Barratt
Sep 25, 2007·Animal Reproduction Science·P I GilL C Giojalas

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Citations

Nov 13, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Ichiro TaniiHideki Fuse
Mar 28, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·H A GuidobaldiL C Giojalas
Jul 19, 2011·Asian Journal of Andrology·Cecilia Soledad BlenginiLaura Cecilia Giojalas
Oct 3, 2013·Protoplasma·Hitoshi Sugiyama, Douglas E Chandler
Dec 10, 2009·PloS One·Maria E TevesLaura C Giojalas
Apr 7, 2010·The Journal of Clinical Investigation·Masahito IkawaMasaru Okabe
May 14, 2010·Biology of Reproduction·Haixin Chang, Susan S Suarez
Oct 27, 2010·PloS One·Tania E Saez LancellottiMiguel W Fornés
Jan 11, 2012·Journal of Visualized Experiments : JoVE·Lindsey A BurnettDouglas E Chandler
Mar 13, 2014·PloS One·Diego Rafael UñatesLaura Cecilia Giojalas
Nov 21, 2013·European Journal of Cell Biology·Juan P ZaninRoberto A Rovasio
May 26, 2011·Molecular Human Reproduction·Manabu Yoshida, Kaoru Yoshida
Jun 5, 2013·Molecular Human Reproduction·L V GaticaL C Giojalas
Jun 7, 2013·Molecular Human Reproduction·Stephen Publicover
Sep 2, 2011·Médecine sciences : M/S·Christophe ArnoultPierre F Ray
Feb 14, 2016·Developmental Biology·Florenza A La SpinaMariano G Buffone
Feb 10, 2016·Saudi Journal of Biological Sciences·Noraina Mohd BakriKhairul Osman
Nov 10, 2009·Fertility and Sterility·Maria Eugenia TevesLaura Cecilia Giojalas
Aug 1, 2012·Reproduction in Domestic Animals = Zuchthygiene·T Fair, P Lonergan
Jun 22, 2011·Molecular Reproduction and Development·Lindsey A BurnettDouglas E Chandler
Dec 25, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Sung Min HanMichael A Miller
Sep 30, 2015·The Journal of Cell Biology·Juan I ErnestoPatricia S Cuasnicú
Oct 20, 2011·Developmental Biology·Lindsey A BurnettDouglas E Chandler
Jun 6, 2016·Theriogenology·Julie LamyMarie Saint-Dizier
Jan 6, 2017·Scientific Reports·Nana KongMeijia Zhang
Apr 14, 2016·Reproductive Sciences·Aideé Saray López-Torres, Mayel Chirinos
Nov 11, 2017·Nature Communications·Guglielmo SaggioratoJens Elgeti
Jul 16, 2017·Reproduction : the Official Journal of the Society for the Study of Fertility·S Gimeno-MartosR Pérez-Pé
Mar 10, 2015·Reproduction, Fertility, and Development·Hilde NelisKatrien Smits
Sep 4, 2015·Biology of Reproduction·Karine ReynaudSylvie Chastant-Maillard
Aug 8, 2020·Biology of Reproduction·Gagandeep Kaur Gahlay, Neha Rajput
Mar 23, 2017·Advanced Materials·Veronika MagdanzOliver G Schmidt
Feb 9, 2017·Molecular Reproduction and Development·Hector A GuidobaldiLaura C Giojalas
Apr 14, 2019·Scientific Reports·Ayelén Moreno-IrustaLaura C Giojalas

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Methods Mentioned

BETA
immunoprecipitation
immunoprecipitation assay
electrophoresis
light microscopy

Software Mentioned

Virtualdub
SpermTrack
SigmaStat

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