PMID: 6160582Oct 1, 1980Paper

Progesterone inhibition of uterine nuclear estrogen receptor: dependence on RNA and protein synthesis

Proceedings of the National Academy of Sciences of the United States of America
R W Evans, W W Leavitt

Abstract

Progesterone treatment was found previously to reduce nuclear estrogen receptor (Re) rapidly (2-4 hr) in the hamster uterus in vivo. The present study was done to determine whether this inhibitory effect of progesterone on uterine nuclear Re could be demonstrated in vitro ad whether progesterone action was dependent on RNA and protein synthesis. A uterine strip system was used in which estradiol pretretment caused cytosol Re translocation to the nucleus and increased synthesis of cytosol progesterone receptor (Rp) during a 16-hr incubation. When progesterone was added to the medium 4 hr before the end of the incubation, cytosol Rp was depleted and nuclear Re was greatly reduced. Further experiments done with actinomycin D, puromycin, and cycloheximide indicated that the progesterone-induced loss of uterine nuclear Re was dependent on RNA and protein synthesis. These results suggest that progesterone reduced nuclear Re through a mechanism involving Rp translocation and the induction of RNA and protein synthesis, a product of which is active in degrading or otherwise inactivating nuclear Re.

References

Jan 1, 1975·Annual Review of Physiology·R M Brenner, N B West
Mar 11, 1977·Annals of the New York Academy of Sciences·J H ClarkE J Peck
Mar 11, 1977·Annals of the New York Academy of Sciences·W W LeavittT C Allen
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Jan 1, 1971·Annual Review of Microbiology·S Pestka
Apr 1, 1969·Steroids·E V JensenE R DeSombre

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Citations

Jan 29, 1982·Biochemical and Biophysical Research Communications·R G MacDonaldW W Leavitt
Aug 1, 1986·Journal of Steroid Biochemistry·A Takeda, W W Leavitt
Oct 1, 1992·Theriogenology·E D WatsonH G Zanecosky
Oct 1, 1983·Molecular and Cellular Endocrinology·R G MacDonaldW W Leavitt
Jun 1, 1989·Molecular and Cellular Endocrinology·E J SmanikV B Mahesh
May 4, 1983·Biochimica Et Biophysica Acta·W C OkuliczW W Leavitt
Jun 2, 2011·Journal of Molecular Endocrinology·M Bertan YilmazSerdar E Bulun
Mar 27, 2010·General and Comparative Endocrinology·Pia Bagamasbad, Robert J Denver
Jan 1, 1986·Annals of the New York Academy of Sciences·W W LeavittR G MacDonald
Nov 15, 1983·Biochemical and Biophysical Research Communications·A J SymsR G Smith
Jan 5, 2000·Physiological Reviews·G D NiswenderE W McIntush

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