Progesterone Inhibits Apoptosis in Fetal Membranes by Altering Expression of Both Pro- and Antiapoptotic Proteins

Reproductive Sciences
Yuguang WangErrol R Norwitz

Abstract

Progesterone supplementation prevents preterm birth (PTB) in some high-risk women, but its mechanism of action is unknown. One-third of PTB is associated with preterm premature rupture of membranes (PPROMs). We have previously shown that progesterone inhibits basal and Tumor Necrosis Factor (TNF) α-induced apoptosis in an explant model of human fetal membranes. This study investigates the molecular mechanisms responsible for progesterone-mediated inhibition of apoptosis in fetal membranes. Human fetal membranes were collected at elective cesarean at term (no labor, no infection; n = 6), washed, and pretreated with/without progesterone (125 ng/mL) for 24 hours. Thereafter, membranes were treated with/without TNFα (50 ng/mL) and/or progesterone for 48 hours, harvested, and homogenized. Apoptosis was determined by evaluating caspase-3, -8, and -9 activities. Expression of pro- BH3 interacting domain death against, Bc1-2 associated X protein (BID, BAX) and antiapoptotic proteins (X-linked inhibitor of apoptosis protein [XIAP], Bcl-2, FLICE inhibitory protein [FLIP]) were measured by Western blot. TNFα increased apoptosis (measured by caspase-3, -8, and -9 activities) in fetal membranes, and this effect was abrogated by progesterone...Continue Reading

References

Feb 1, 1995·American Journal of Obstetrics and Gynecology·R MenonS J Fortunato
Oct 1, 1994·American Journal of Reproductive Immunology : AJRI·S J FortunatoT W Lyden
May 31, 2002·Journal of Assisted Reproduction and Genetics·Ramkumar MenonStephen J Fortunato
Nov 16, 2002·Lancet·Michael M Slattery, John J Morrison
Nov 26, 2002·International Journal of Gynaecology and Obstetrics : the Official Organ of the International Federation of Gynaecology and Obstetrics·A Shobokshi, M Shaarawy
Jun 13, 2003·The New England Journal of Medicine·Paul J MeisUNKNOWN National Institute of Child Health and Human Development Maternal-Fetal Medicine Units Network
Dec 28, 2006·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Anna K Sfakianaki, Errol R Norwitz
Apr 4, 2007·American Journal of Obstetrics and Gynecology·Tamas Zakar, Frank Hertelendy
Aug 3, 2007·The New England Journal of Medicine·Eduardo B FonsecaUNKNOWN Fetal Medicine Foundation Second Trimester Screening Group
Dec 14, 2007·American Journal of Perinatology·Ronald B GeorgeAmy P Murtha
Jan 8, 2008·Lancet·Robert L GoldenbergRoberto Romero
Oct 16, 2009·The New England Journal of Medicine·Richard S HotchkissPaul E Swanson
Sep 28, 2010·American Journal of Obstetrics and Gynecology·Christian M BrieryJohn C Morrison
Oct 5, 2010·Reproductive Sciences·Sam MesianoErrol R Norwitz
Aug 23, 2011·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Suzanne ColemanNiki Istwan
Sep 22, 2012·Obstetrics and Gynecology·UNKNOWN Committee on Practice Bulletins—Obstetrics, The American College of Obstetricians and Gynecologists
May 21, 2014·Reproductive Sciences·Sophia M R LannonKristina M Adams Waldorf
Jun 14, 2015·American Journal of Obstetrics and Gynecology·Deepak KumarJohn J Moore
May 26, 2016·Nature Communications·Lubna NadeemStephen Lye
Aug 16, 2017·Seminars in Perinatology·Ramkumar Menon, Lauren S Richardson

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Citations

Apr 26, 2020·The Journal of Perinatal & Neonatal Nursing·Eleanor E WadeAndrew S Thagard
May 16, 2021·Cellular Signalling·Yuliya FakhrDenise G Hemmings
Jun 10, 2021·Reproductive Sciences·Banghyun LeeHee Joong Lee

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