Progesterone Inhibits Endothelial Cell Migration Through Suppression of the Rho Activity Mediated by cSrc Activation

Journal of Cellular Biochemistry
Tong-Sheng LeeWen-Sen Lee

Abstract

We previously showed that progesterone (P4) could inhibit the proliferation of human umbilical venous endothelial cells (HUVECs) through the p53-dependent pathway. In the present study, we further demonstrated that P4 at physiologic levels (5-500 nM) concentration-dependently inhibited migration of HUVECs. This effect was blocked by pre-treatment with the P4 receptor (PR) agonist-antagonist, RU486, suggesting that the P4-induced migration inhibition in HUVECs was through the PR-mediated signaling pathway. Western blot analyses demonstrated that the levels of RhoA and Rac-1 protein were reduced in the P4-treated HUVECs. P4 also inhibited the membrane translocation of RhoA and Rac-1 protein. Moreover, the P4-induced migration inhibition in HUVECs was prevented by over-expression of the constitutively active RhoA construct (RhoA V14). However, pre-treatment with the ROCK (a kinase associated with RhoA for transducing RhoA signaling) inhibitor, Y27632, abolished the over-expression of RhoA-induced prevention effect on the P4-induced migration inhibition in HUVECs. These data suggest that the inhibition of Rho GTPases might account for the P4-induced migration inhibition of HUVECs. Pre-treatment with the cSrc inhibitor, PP2, prevent...Continue Reading

References

Oct 1, 1995·Cardiovascular Research·H Abedi, I Zachary
Apr 17, 1997·Nature·W Risau
Jul 1, 1997·Trends in Biochemical Sciences·F BussolinoG Persico
Sep 1, 1997·Nature Medicine·W S LeeE Haber
Feb 7, 1998·Science·A Hall
Jun 2, 1998·Cellular Signalling·M C Seabra
Jan 16, 1999·The Journal of Biological Chemistry·F VázquezM L Iruela-Arispe
Jun 30, 2000·Current Biology : CB·W T ArthurK Burridge
Apr 10, 2002·American Journal of Physiology. Cell Physiology·Michael Papetti, Ira M Herman
Mar 15, 2003·Nature Reviews. Cancer·John D Hood, David A Cheresh
Jun 12, 2003·World Journal of Gastroenterology : WJG·Manoj Kumar Gupta, Ren-Yi Qin
Mar 24, 2004·Critical Reviews in Oncology/hematology·Georges BellonArnaud Robinet
Apr 13, 2004·Molecular Biology of the Cell·Celeste M NelsonChristopher S Chen
Jul 1, 2004·Journal of Cell Science·Matthew WherlockHarry Mellor
Oct 27, 2004·Experimental Cell Research·Ann P Wheeler, Anne J Ridley
Nov 5, 2004·Journal of Cellular Biochemistry·Kaspar KeledjianNatasha Kyprianou
Dec 27, 2005·Current Cancer Drug Targets·Lee Ann Cohen, Jun-Lin Guan
Dec 27, 2005·Cellular and Molecular Life Sciences : CMLS·S J McTaggart
Dec 19, 2006·Molecular Cancer Therapeutics·Pei-Yin HoWen-Sen Lee
Oct 14, 2008·Cellular and Molecular Life Sciences : CMLS·S-P HsuW-S Lee
Aug 17, 2010·Current Opinion in Cell Biology·Elizabeth S Harris, W James Nelson
Jan 3, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ming-Hsien ChienShun-Fa Yang

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Citations

Apr 6, 2016·Endocrine Research·Claudia VanettiMaria Grazia Cattaneo
Jan 28, 2017·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Wei-Jin ZhangQiao-Bing Huang
Apr 26, 2016·Oncology Reports·Bo LiuLike Zhang
Sep 11, 2019·European Journal of Nutrition·Hui-Chen WangWen-Sen Lee
Mar 9, 2017·Molecular Cancer Therapeutics·Bing Z CarterMichael Andreeff
Mar 20, 2021·Cardiovascular Research·Andrea CignarellaMatthias Barton

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