Progesterone inhibits superoxide radical production by mononuclear phagocytes in pseudopregnant rats

Endocrinology
N SuginoH Kato

Abstract

This study investigated the changes in superoxide radical production by mononuclear phagocytes in the corpus luteum (CL) during pseudopregnancy in rats. Activity of superoxide radical production was determined by the conversion of nitro blue tetrazolium (NBT) to blue formazan deposit. Rats received 10 mg NBT via the abdominal aorta on day 3, 7, or 13 of pseudopregnancy and were autopsied 1 min later to prepare the histological sections. The cells with blue formazan deposits (NBT-positive cells) in the CL were scarce on days 3 and 7 of pseudopregnancy and significantly increased on day 13 of pseudo-pregnancy. On the other hand, simultaneous administration of 100 micrograms phorbol 12-myristate 13-acetate, which activates mononuclear phagocytes to produce superoxide radical, significantly increased the numbers of NBT-positive cells in the CL on day 7 of pseudopregnancy, but not in the CL on day 3 or 13 of pseudopregnancy. To study the possibility that superoxide radical production by mononuclear phagocytes is inhibited by progesterone on day 7 of pseudopregnancy, peritoneal mononuclear phagocytes prepared on day 7 or 13 of pseudopregnancy were preincubated with 10, 50, or 100 ng/ml progesterone for 6 h and then stimulated with ph...Continue Reading

Citations

Dec 3, 2014·The Journal of Reproduction and Development·Rei HirataKiyoshi Okuda
Jul 13, 2001·Menopause : the Journal of the North American Menopause Society·M A Gómez-ZubeldiaJ C Millán
Mar 7, 2005·Reproductive Medicine and Biology·Norihiro Sugino
Jun 20, 2008·Molecular Reproduction and Development·H KliemD Schams
Dec 20, 2008·Pakistan Journal of Biological Sciences : PJBS·Leila Roushangar, Jafar Soleimani Rad
Aug 1, 1998·Biology of Reproduction·F GaytánJ E Sánchez-Criado
May 20, 2000·Annals of the New York Academy of Sciences·A MakrigiannakisC Coutifaris

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