Progesterone is a suppressor of apoptosis in bovine luteal cells

Biology of Reproduction
K OkudaD J Skarzynski

Abstract

Progesterone is suggested to be a suppressor of apoptosis in bovine luteal cells. Fas antigen (Fas) is a cell surface receptor that triggers apoptosis in sensitive cells. Furthermore, apoptosis is known to be controlled by the bcl-2 gene/protein family and caspases. This study was undertaken to determine whether intraluteal progesterone (P4) is involved in Fas L-mediated luteal cell death in the bovine corpus luteum (CL) in vitro. Moreover, we studied whether an antagonist of P4 influences gene expression of the bcl-2 family and caspase-3 and the activity of caspase-3 in the bovine CL. Luteal cells obtained from the cows in the midluteal phase of the estrous cycle (Days 8-12 of the cycle) were exposed to a specific P4 antagonist (onapristone [OP], 10(-4) M) with or without 100 ng/ml Fas L. Although Fas L alone did not show a cytotoxic effect, treatment of the cells with OP alone or in combination with Fas L resulted in killing of 30% and 45% of the cells, respectively (P <0.05). DNA fragmentation was observed in the cells treated with Fas L in the presence of OP. The inhibition of P4 action by OP increased the expression of Fas mRNA (P <0.01); however, it did not affect bax or bcl-2 mRNA expression (P >0.05). Moreover, OP stimu...Continue Reading

References

Jun 1, 1992·Biology of Reproduction·K OkudaD Schams
Nov 1, 1986·Journal of Steroid Biochemistry·W ElgerR Rohde
Mar 10, 1995·Science·S Nagata, P Golstein
Jan 1, 1993·Endocrinology·J L JuengelM F Smith
Feb 7, 1997·Cell·S Nagata
May 1, 1997·Molecular Reproduction and Development·K SakamakiS Yonehara
Sep 1, 1996·Reviews of Reproduction·J L Tilly
Apr 30, 1999·Physiological Reviews·J A McCrackenJ C Lamsa
Dec 28, 1999·Biology of Reproduction·S L VickersS M Quirk
Jun 22, 2000·Biology of Reproduction·S M QuirkR G Cowan
Jun 28, 2001·Reproduction : the Official Journal of the Society for the Study of Fertility·M G PetroffJ L Pate
Aug 30, 2001·Cell Death and Differentiation·E LongX Zhao
Mar 1, 2002·Biology of Reproduction·Hiroaki TaniguchiKiyoshi Okuda
Nov 14, 2003·Reproductive Biology and Endocrinology : RB&E·Kiyoshi Okuda, Ryosuke Sakumoto
May 1, 1996·Theriogenology·W Hansel, G Cain
May 1, 1996·Theriogenology·J L Pate

❮ Previous
Next ❯

Citations

Sep 29, 2011·Cardiovascular Toxicology·Stephen MorrissyQin M Chen
Jun 14, 2005·Domestic Animal Endocrinology·Dariusz J SkarzynskiKiyoshi Okuda
Feb 3, 2006·Biology of Reproduction·John J Peluso
Feb 26, 2011·Reproductive Biology and Endocrinology : RB&E·Anna J KorzekwaDariusz J Skarzynski
Feb 24, 2006·The Journal of Reproduction and Development·Anna J KorzekwaDariusz J Skarzynski
Nov 7, 2007·The Journal of Reproduction and Development·Norihiro Sugino, Kiyoshi Okuda
Feb 7, 2013·The Journal of Reproduction and Development·Syota KawaguchiKiyoshi Okuda
Jan 30, 2013·The Journal of Reproduction and Development·Syota KawaguchiKiyoshi Okuda
Feb 17, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Nicola Beindorff, Almuth Einspanier
Aug 31, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Marina C PeluffoMarta Tesone
Dec 18, 2012·Reproduction : the Official Journal of the Society for the Study of Fertility·Susan M QuirkRebecca M Harman
Dec 10, 2009·The Journal of Reproduction and Development·Ryosuke SakumotoFred Sinowatz
May 15, 2012·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Nicel TasdemirGulnur Ozaksit
Jul 30, 2011·Chronobiology International·Guiyan ChuMasa-Aki Hattori
Oct 16, 2012·Reproductive Biomedicine Online·Kaïs H Al-GuboryNorihiro Sugino
Mar 31, 2015·The Journal of Reproduction and Development·Mansour AboelenainMasashi Takahashi
Dec 3, 2014·The Journal of Reproduction and Development·Rei HirataKiyoshi Okuda
Aug 4, 2009·Reproduction in Domestic Animals = Zuchthygiene·A KorzekwaD J Skarzynski
Feb 20, 2008·Reproduction in Domestic Animals = Zuchthygiene·A J KorzekwaD J Skarzynski
Jul 20, 2007·Reproduction in Domestic Animals = Zuchthygiene·G Ferreira-DiasC M P Rodrigues
Jun 20, 2008·Molecular Reproduction and Development·H KliemD Schams
Aug 24, 2010·Aging Cell·Stephen MorrissyQin M Chen
Aug 24, 2013·Reproduction in Domestic Animals = Zuchthygiene·D J SkarzynskiR Meidan
Mar 26, 2013·Reproduction in Domestic Animals = Zuchthygiene·L M ZorrillaJ E Gadsby
Nov 30, 2018·The Journal of Reproduction and Development·Hiroki HasegawaKiyoshi Okuda
Jan 26, 2008·Biology of Reproduction·Junichi KomiyamaKiyoshi Okuda
May 9, 2008·Biology of Reproduction·Anom BowolaksonoKiyoshi Okuda

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
PCR
PCRs
ELISA

Software Mentioned

Blast
Primer3
GraphPad
GraphPAD PRISM
GENETYX

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptotic Caspases

Apoptotic caspases belong to the protease enzyme family and are known to play an essential role in inflammation and programmed cell death. Here is the latest research.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.