Progesterone receptor does not form oligomeric (8S), non-DNA-binding complex in intact cell nuclei

Journal of Cellular Biochemistry
A PekkiP Tuohimaa

Abstract

We raised a polyclonal antibody, alpha D, against a synthetic peptide (amino acids 522-535) of chicken progesterone receptor (PR). The sequence is located between the DNA-binding domain and the hormone-binding domain in the region within the sequences required for stability of the oligomeric form of PR. In the immunoblot, alpha D reacted with both A and B forms of PR. In the sucrose gradient and dot-blot the antibody did not recognize the so-called 8S form of PR, which is an oligomeric complex of PR and other proteins. When the oligomeric complex was dissociated by salt treatment, the antibody recognized the resulting 4S form of PR. This would suggest that the epitope is masked in the 8S form of PR and exposed in the 4S form. To study whether a similar complex exists in vivo, we used the antibody for immunohistochemistry. Two different fixation techniques were employed, freeze-drying-vapor fixation and liquid fixation. In the animals not treated with progesterone, intensive nuclear staining was detected independent of the fixation technique. When receptor from similarly treated animals was analyzed by sucrose gradient, all of the receptor molecules were in the oligomeric complex (8S). Ligand binding is known to promote a dissoc...Continue Reading

References

Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·D F Smith, D O Toft
May 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H Gronemeyer
Jul 9, 1992·Nature·H WiechU Jakob
Jan 1, 1991·Annual Review of Genetics·H Gronemeyer
Aug 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A K Pekki
May 1, 1990·Endocrine Reviews·M A Carson-JuricaB W O'Malley
Jun 1, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·T K Joensuu
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J M JeltschP Chambon
Oct 1, 1986·Endocrinology·W P SullivanD O Toft
Jan 1, 1989·Journal of Steroid Biochemistry·M A Carson-JuricaB W O'Malley
Apr 1, 1989·Journal of Steroid Biochemistry·F Auricchio
Aug 1, 1989·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A Pekki, P Tuohimaa
Sep 1, 1989·Molecular and Cellular Biology·S L KostD O Toft
Aug 1, 1987·Molecular Endocrinology·O M ConneelyB W O'Malley
Feb 1, 1988·Journal of Cellular Biochemistry·W P SullivanD O Toft
Aug 1, 1984·Molecular and Cellular Endocrinology·J M Renoir, J Mester
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·K I KangM G Catelli
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P TuohimaaT Ylikomi

❮ Previous
Next ❯

Citations

Aug 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·H SyväläP Tuohimaa
Oct 16, 2001·Cell Stress & Chaperones·M HaverinenT Ylikomi
Mar 11, 2015·International Journal of Cancer. Journal International Du Cancer·Gisela I MazairaMario D Galigniana
Feb 24, 2001·Progress in Histochemistry and Cytochemistry·S Yamashita
Feb 24, 1997·Biochemical and Biophysical Research Communications·H SyväläP Tuohimaa

❮ Previous
Next ❯

Related Concepts

Related Feeds