Abstract
Breast cancer resistance protein (BCRP) plays a major role in multidrug resistance (MDR). Sequence analysis reveals there is a novel progesterone response element (PRE) in the BCRP promoter, suggesting progesterone receptor (PR) may have a function in the regulation of BCRP expression. We examined the expressions of BCRP, PR, estrogen receptor α (ERα), androgen receptor (AR) and Her-2 in 95 breast cancer samples by immunohistochemistry. Then, to identify the role of PR in the regulation of BCRP expression, two constructs encoding full length BCRP cDNA driven by putative PRE promoter or constitutive CMV promoter were transfected into MCF-7 and MDA-MB-231, respectively. Reverse transcription-polymerase chain reaction (RT-PCR) and western blotting were used to detect the expression of BCRP. Further electrophoretic mobility shift assay (EMSA) was used to verify the nuclear protein-DNA specific binding. We innovatively found that the expression of BCRP negatively related with that of PR and ERα in breast cancer by immunohistochemistry. While at a cellular level, after being treated by progesterone and 17β-estradiol, BCRP mRNA and protein levels were significantly reduced in a concentration-dependent manner in MCF-7/P-BCRP cells with...Continue Reading
References
Jan 1, 1993·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·M H LiberatoM M Brentani
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·L A DoyleD D Ross
May 20, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·J M HarveyD C Allred
Dec 8, 2000·Steroids·S C Hewitt, K S Korach
Jan 24, 2002·Journal of Bioenergetics and Biomembranes·G D KruhM G Belinsky
Mar 21, 2002·Nature Reviews. Cancer·Michael M GottesmanSusan E Bates
Sep 23, 2003·The Journal of Urology·Julio E DiestraMiguel A Izquierdo
Oct 8, 2003·The Oncologist·Gregory D LeonardSusan E Bates
Feb 3, 2004·Current Medicinal Chemistry. Anti-cancer Agents·B Han, J-T Zhang
Feb 20, 2004·Cancer Research·Pui Lai Rachel EeWilliam T Beck
Mar 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kiyotaka YohAtsushi Ochiai
Jul 2, 2005·Cancer Letters·Patricia A FetschSusan E Bates
Aug 3, 2005·Cancer Research·Wendy J HussGary J Smith
Dec 15, 2005·American Journal of Physiology. Endocrinology and Metabolism·Honggang WangQingcheng Mao
Feb 27, 2007·Cancer Metastasis Reviews·Robert W RobeySusan E Bates
Sep 20, 2007·Oncology·Yuhua ZhangDeling Yin
Nov 29, 2007·Molecular Pharmacology·Honggang WangQingcheng Mao
Dec 10, 2008·Biochemistry·Honggang WangQingcheng Mao
Jan 21, 2010·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Laura BagliettoGraham G Giles
May 13, 2010·The Journal of Pharmacology and Experimental Therapeutics·A M S HartzB Bauer
May 13, 2010·Cancer Research·Angel M CarcabosoClinton F Stewart
Sep 4, 2010·Current Drug Metabolism·Zhanglin NiQingcheng Mao
Aug 13, 2011·Breast Cancer Research and Treatment·Sibylle LoiblCarsten Denkert
Dec 31, 2011·Türk patoloji dergisi·Maha Arafah
Citations
Dec 25, 2012·Yi chuan = Hereditas·Xin-Gang WuQian Huang
Jan 8, 2013·Expert Opinion on Drug Metabolism & Toxicology·Rebecca Schnepf, Oliver Zolk
Oct 10, 2013·The FEBS Journal·Weiwei LiGengyin Zhou
Jan 28, 2014·Biomaterials·Xinxin ZhangYong Gan
Sep 22, 2017·Current Medicinal Chemistry·Juan Pablo RigalliJohanna Weiss
Feb 14, 2020·Genes·Yujuan ZhangLiping Wang
Feb 23, 2020·Expert Opinion on Drug Metabolism & Toxicology·Ludwik Gorczyca, Lauren M Aleksunes
Apr 23, 2020·International Journal of Oncology·Xiumei WangYong Xu
Jul 23, 2020·Frontiers in Oncology·Jing HuPeng Su
Mar 22, 2014·Molecular and Clinical Oncology·En-Qi QiaoJi-Hai Tang
Jan 10, 2021·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Milica NedeljkovićNikola Tanić
Dec 15, 2020·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Ian D KerrIngrid C Gelissen
Apr 30, 2021·Frontiers in Oncology·Jing ZhangXiaojuan Wu