PMID: 6207179Oct 1, 1984Paper

Progesterone receptor in the chick oviduct: an immunohistochemical study with antibodies to distinct receptor components

The Journal of Cell Biology
J M GascE E Baulieu

Abstract

We performed immunohistochemical studies of chicken oviduct after different fixation procedures, by using antibodies against the progesterone receptor: polyclonal antibodies IgG-G3 against the "8S" form (an oligomere containing progesterone-binding and nonprogesterone-binding units), polyclonal antibodies IgG-RB against the progesterone-binding B subunit, and monoclonal BF4 against the non-progesterone-binding 90,000-mol-wt protein component. Chickens were immature animals with or without estrogen priming, and with or without progesterone treatment. The antibodies were revealed by means of an immunoperoxidase technique that used the avidin-biotin-peroxidase complex, and controls were performed by presaturation of antibodies with the purified 8S-progesterone receptor, the B subunit, and 90,000-mol-wt protein. The progesterone receptor was detected not only in well-characterized target tissues, i.e., in glands and luminal epithelium, but also in stromal cells (some displayed the strongest reaction), in mesothelium, and in fibers of smooth muscles. Only in cell nuclei, whether or not the animals received an injection of progesterone was an antigen revealed corresponding to the B subunit (and/or to the A subunit, because there is i...Continue Reading

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Citations

Jan 1, 1990·The International Journal of Biochemistry·J F SavouretE Milgrom
Jan 1, 1986·Journal of Steroid Biochemistry·G L Greene, M F Press
Jan 1, 1986·Journal of Steroid Biochemistry·L A van der Walt, J L Wittliff
Sep 1, 1986·Journal of Steroid Biochemistry·R J King
Aug 1, 1986·Journal of Steroid Biochemistry·T F Ogle
Jan 1, 1987·Journal of Steroid Biochemistry·J IsolaP Tuohimaa
Jan 1, 1987·Journal of Steroid Biochemistry·J M Gasc, E E Baulieu
Jan 1, 1988·Journal of Steroid Biochemistry·T Ylikomi, P Tuohimaa
Jan 1, 1988·Journal of Steroid Biochemistry·W DietrichE Heise
Jan 1, 1989·Journal of Steroid Biochemistry·M Kaufman, L Pinsky
Jan 1, 1989·Journal of Steroid Biochemistry·T K Joensuu, P J Tuohimaa
Dec 1, 1989·Journal of Steroid Biochemistry·S E RundleJ W Funder
May 1, 1989·Journal of Steroid Biochemistry·L DufreneE E Baulieu
Jun 1, 1986·Molecular and Cellular Endocrinology·H Gronemeyer, M V Govindan
Jul 1, 1987·Molecular and Cellular Endocrinology·T J YlikomiP J Tuohimaa
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Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P TuohimaaT Ylikomi
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Jan 1, 1990·Annals of the New York Academy of Sciences·E E BaulieuC Radanyi
Jan 1, 1991·Annals of the New York Academy of Sciences·E E Baulieu

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