Progesterone receptor transcriptome and cistrome in decidualized human endometrial stromal cells

Endocrinology
Erik C MazurFrancesco J DeMayo

Abstract

Decidualization is a complex process involving cellular proliferation and differentiation of the endometrial stroma that is required to establish and support pregnancy. Progesterone acting via its nuclear receptor, the progesterone receptor (PGR), is a critical regulator of decidualization and is known to interact with certain members of the activator protein-1 (AP-1) family in the regulation of transcription. In this study, we identified the cistrome and transcriptome of PGR and identified the AP-1 factors FOSL2 and JUN to be regulated by PGR and important in the decidualization process. Direct targets of PGR were identified by integrating gene expression data from RNA sequencing with the whole-genome binding profile of PGR determined by chromatin immunoprecipitation followed by deep sequencing (ChIP-seq) in primary human endometrial stromal cells exposed to 17β-estradiol, medroxyprogesterone acetate, and cAMP to promote in vitro decidualization. Ablation of FOSL2 and JUN attenuates the induction of 2 decidual marker genes, IGFBP1 and PRL. ChIP-seq analysis of genomic binding revealed that FOSL2 is bound in proximity to 8586 distinct genes, including nearly 80% of genes bound by PGR. A comprehensive assessment of the PGR-depen...Continue Reading

References

Jan 1, 1990·The International Journal of Biochemistry·J F SavouretE Milgrom
Jan 1, 1994·Annual Review of Biochemistry·M J Tsai, B W O'Malley
Mar 1, 1994·The Journal of Clinical Endocrinology and Metabolism·I P RyanR N Taylor
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·A M BambergerH M Schulte
Jun 25, 1998·Molecular and Cellular Biology·S M Jacobs-HelberS T Sawyer
May 15, 2002·Reviews in Endocrine & Metabolic Disorders·Hugh S Taylor
Jul 9, 2002·Molecular and Cellular Biology·Suzanne E WardellDean P Edwards
Apr 6, 2006·Cancer Research·Nicole S SpoelstraJennifer K Richer
Oct 3, 2006·Nature Genetics·Jason S CarrollMyles Brown
Jun 5, 2007·PLoS Genetics·Chin-Yo LinEdison T Liu
Oct 26, 2007·Seminars in Reproductive Medicine·Birgit GellersenJan J Brosens
May 20, 2008·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Meenakshi SinghJennifer K Richer
Jul 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Robert EferlErwin F Wagner
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Jan 10, 2009·Nature Protocols·Da Wei HuangRichard A Lempicki
Mar 6, 2009·Genome Biology·Ben LangmeadSteven L Salzberg
Mar 18, 2009·Bioinformatics·Cole TrapnellSteven L Salzberg
Apr 3, 2009·The EMBO Journal·Willem-Jan WelborenHendrik G Stunnenberg
Dec 26, 2009·Reproductive Biology and Endocrinology : RB&E·Karin TammMadis Metsis
Feb 4, 2010·Molecular and Cellular Biology·Hilary E WadeDonald P McDonnell
Feb 26, 2011·Annals of the Rheumatic Diseases·Helia B SchonthalerErwin F Wagner
Mar 11, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·J C WarningJ M Morris
Aug 24, 2011·Genome Biology·Tao LiuX Shirley Liu
Sep 21, 2011·Endocrine Journal·Toshio MatsumotoShinsuke Kido
Sep 29, 2011·Molecular and Cellular Biology·Lindsey N KentMichael J Soares
Nov 26, 2011·Molecular and Cellular Endocrinology·Margeaux Wetendorf, Francesco J DeMayo
Apr 28, 2012·Proceedings of the National Academy of Sciences of the United States of America·Sanhita Ray, Jeffrey W Pollard
May 29, 2012·Molecular Endocrinology·Cory A RubelFrancesco J DeMayo
Feb 6, 2013·Medical Molecular Morphology·Takeshi KajiharaOsamu Ishihara

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Citations

Feb 14, 2016·Human Reproduction·K KrjutškovJ Kere
Jan 26, 2016·American Journal of Reproductive Immunology : AJRI·Arpita S BhurkeMilan K Bagchi
Sep 18, 2015·PLoS Genetics·Tae Hoon KimJae-Wook Jeong
Jul 7, 2016·Journal of Molecular Biology·Sandra L GrimmDean P Edwards
Oct 18, 2016·American Journal of Human Genetics·Katelyn M Mika, Vincent J Lynch
Dec 21, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Pavle VrljicakSascha Ott
Jan 8, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·San-Pin WuFrancesco J DeMayo
Oct 31, 2019·The Journal of Clinical Endocrinology and Metabolism·Ru-Pin Alicia ChiFrancesco DeMayo
Sep 1, 2020·Molecular Human Reproduction·C D HalariP K Lala
Dec 1, 2017·Biology of Reproduction·Maria M SzwarcJohn P Lydon
Jul 17, 2018·Biology of Reproduction·Koji Yoshinaga
Feb 16, 2021·Frontiers in Cell and Developmental Biology·Sarah L HardenYie Hou Lee
Jan 23, 2021·Journal of Clinical Medicine·Hiromi MurataHidetaka Okada
Mar 7, 2021·Scientific Reports·Jarrod McKennaPeter Temple-Smith
May 1, 2018·Trends in Endocrinology and Metabolism : TEM·San-Pin WuFrancesco J DeMayo
Apr 7, 2021·Molecular Human Reproduction·Nawrah KhaderJennifer A Mitchell
May 7, 2021·The Journal of Clinical Endocrinology and Metabolism·Stefania SalsanoFrancisco Domínguez

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