PMID: 8982272Dec 13, 1996Paper

Progesterone triggers the rapid activation of phospholipase D in the amphibian oocyte plasma membrane when initiating the G2/M transition

Biochimica Et Biophysica Acta
A B KostellowG A Morrill

Abstract

Previous reports indicate that, in the Rana pipiens oocyte, progesterone triggers a rapid rise in 1,2-diacylglycerol (DAG) derived from phosphatidylcholine (PC) in the plasma membranes. This DAG transient, which appears and is terminated within 60-90 s, is derived both from a phospholipase which we assumed to be phospholipase C and from sphingomyelin (SM) synthase. We now find that progesterone stimulates PC and DAG turnover primarily via the phospholipase D (PLD) and phosphatidic acid phosphohydrolase (PAP) pathways as well as via the SM-ceramide pathway. Rana oocytes were prelabeled with [3H]choline chloride under conditions in which about 70% is incorporated into PC of the plasma membrane of the intact oocyte or with [3H]lysoplatelet activating factor (1-O-octadecyl-sn-glycero-3-phosphocholine, lysoPAF) which is selectively incorporated into plasma membrane PC. Progesterone induced the release of [3H]choline from intact oocytes into the medium within 60-90 s. This choline release was dose-dependent and was not inhibited by a putative PC-specific phospholipase C inhibitor, D609. Progesterone also induced a transient rise in [3H]lysoPAF-derived [3H]DAG within 1-2 min followed by a rise in [3H]PA. In the presence of 20 mM ethan...Continue Reading

References

Jan 1, 1990·Progress in Lipid Research·C Kent
Apr 1, 1991·Trends in Pharmacological Sciences·B S McEwen
Sep 15, 1987·Biochemical and Biophysical Research Communications·A B KostellowG A Morrill
Jun 1, 1966·Journal of Cellular Physiology·G A MorrillD E Watson

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Citations

Aug 18, 2005·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Jorgelina Buschiazzo, Telma Susana Alonso
Jan 1, 2009·Molecular Reproduction and Development·Stephen A Stricker
Mar 6, 1997·Biochemical and Biophysical Research Communications·G A MorrillA Kostellow

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