Prognostic implication of MET overexpression in myxofibrosarcomas: an integrative array comparative genomic hybridization, real-time quantitative PCR, immunoblotting, and immunohistochemical analysis

Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc
Jen-Chieh LeeHsuan-Ying Huang

Abstract

It remains obscure in myxofibrosarcoma about the basis of tumorigenesis, progression, and metastasis. Chromosome 7 gains are common in some sarcomas, including myxofibrosarcoma, whereas the specific oncogenes are yet to be characterized. We performed an integrative study of MET gene at 7q31.2 to elucidate its implication in myxofibrosarcoma. Focused on candidate oncogenes on chromosome 7, 385K array comparative genomic hybridization was used to profile DNA copy number alterations of 12 samples. MET transcript was successfully quantified by real-time RT-PCR for 16 laser-microdissected tumors and two myxofibrosarcoma cell lines (NMFH-1, OH931). MET immunoexpression was assessable in 86 primary localized tumors with follow-up. To analyze endogenous MET expression and activation, NMFH-1 and OH931 cells, both with wild-type MET gene, were subjected to Western blotting and hepatocyte growth factor-treated NMFH-1 cells were evaluated for the kinetics of MET tyrosine phosphorylation. Non-random large-scale gains on 7q were detected in five cases, delineating three recurrent amplicons, 7q21.11-7q21.3, 7q22.1-22.3, and 7q31.1-7q32.3, in which the locus of MET displayed increased copy number, among others. MET mRNA was upregulated in OH93...Continue Reading

References

Apr 1, 1996·The American Journal of Surgical Pathology·T MentzelC D Fletcher
Jan 1, 1997·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·L GuillouJ Costa
Apr 1, 1997·Cancer Genetics and Cytogenetics·A K KrauseJ A Bridge
Jun 22, 2000·Genes, Chromosomes & Cancer·C StockP F Ambros
Nov 1, 2001·Nature Genetics·A M SnijdersD G Albertson
Dec 18, 2001·Cancer·M TarkkanenY S Knuutila
Oct 14, 2003·Oncogene·Sabine MüllerThorsten Melcher
Oct 12, 2004·Biostatistics·Adam B OlshenMichael Wigler
Jun 14, 2005·Trends in Molecular Medicine·Simona CorsoSilvia Giordano
Jun 30, 2005·International Journal of Cancer. Journal International Du Cancer·Selina RaguzErnesto Yagüe
Aug 6, 2005·Cancer Genetics and Cytogenetics·Hiroyuki KawashimaNaoto Endo
Jan 18, 2006·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Stefan M WillemsRaphäel Sciot
Jan 24, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hsuan-Ying HuangChing-Yeh Hsiung
May 3, 2006·Cancer Research·Salvatore PatanèMaria Flavia Di Renzo
Jun 15, 2007·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Etonia Y-T PangNathalie Wong
Aug 4, 2007·Virchows Archiv : an International Journal of Pathology·Mitsuhiko OsakiHisao Ito
Oct 24, 2007·Cancer·Takahiko NakaAlbert Roessner
Dec 21, 2007·Proceedings of the National Academy of Sciences of the United States of America·James BeanWilliam Pao
Feb 26, 2008·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Cristina Migliore, Silvia Giordano
Jul 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Francesca Diomedi-CamasseiCarlo Dominici
Aug 19, 2008·Genes, Chromosomes & Cancer·Patrick C MaRavi Salgia
Aug 30, 2008·Cancer Biology & Therapy·P Sreekanth ReddyKumaravel Somasundaram
Oct 3, 2008·Breast Cancer Research : BCR·D Joseph JerryHaoheng Yan
Dec 17, 2008·Cancer Research·Cristina MiglioreSilvia Giordano
Mar 26, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Joseph Paul EderPatricia M LoRusso
Aug 28, 2009·The Journal of Biological Chemistry·Dongsheng YanLili Tu
Nov 6, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hsuan-Ying HuangChien-Feng Li
Dec 31, 2009·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Zhenfeng DuanFrancis J Hornicek

❮ Previous
Next ❯

Citations

Jun 28, 2012·Sarcoma·Varun DewanRobert Grimer
Jun 20, 2013·Endocrine-related Cancer·Cristina Rodríguez-AntonaMercedes Robledo
Apr 24, 2014·Thyroid : Official Journal of the American Thyroid Association·Veronika MancikovaMercedes Robledo
Jan 1, 2011·Genetics Research International·Jun NishioMasatoshi Naito
Jun 3, 2015·Journal of Surgical Oncology·Makoto EmoriToshihiko Yamashita
Jun 4, 2017·BMJ Case Reports·Richard AntbringAlastair Cj Windsor
Jul 19, 2018·Nature Communications·Koichi OguraTatsuhiro Shibata
Nov 12, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chien-Feng LiHsuan-Ying Huang
Feb 11, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Chien-Feng LiHsuan-Ying Huang
Apr 4, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Hsuan-Ying HuangChien-Feng Li
May 11, 2021·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Jonathan C SlackKyle C Kurek
May 26, 2020·Oncology Research and Treatment·Andrea SambriGiuseppe Bianchi

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiomyopathy

Cardiomyopathy is a disease of the heart muscle, that can lead to muscular or electrical dysfunction of the heart. It is often an irreversible disease that is associated with a poor prognosis. There are different causes and classifications of cardiomyopathies. Here are the latest discoveries pertaining to this disease.