Prognostic Significance and Related Mechanisms of Hexokinase 1 in Ovarian Cancer

OncoTargets and Therapy
Yanqing LiYang Li

Abstract

Ovarian cancer (OC) has the highest mortality among gynecological malignancies. Therefore, it is urgent to explore prognostic biomarkers to improve the survival of OC patients. One of the most prominent metabolic characteristics of cancer is effective glycolysis. Hexokinase 1 (HK1), as the first rate-limiting enzyme in glycolysis, is closely related to cancer progression. However, the role of HK1 in OC remains unclear. The Cancer Genome Atlas (TCGA) database was used to detect the expression of HK1 in OC patients. The chi-squared test was performed to examine the correlations between HK1 and patients' clinical characteristics. Survival analyses were undertaken to determine the relationship between HK1 and patient survival, while the univariate/multivariate Cox model was used to evaluate the role of HK1 in patient prognosis. Gene Set Enrichment Analysis (GSEA) was performed to ascertain the related signaling pathways of HK1. RT-qPCR was implemented to validate the mRNA expression of HK1 in OC cells. MTT was used to detect cell viability after adding 2DG and knocking down HK1 in OC cells. HK1 protein expression was examined by Western blotting. Glucose uptake, lactate production, and ATP assays were undertaken following knockdown...Continue Reading

References

Jan 1, 1995·Reviews of Physiology, Biochemistry and Pharmacology·J E Wilson
Jul 29, 1998·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·S Sebastian, U W Kenkare
Aug 9, 2001·Neoplasia : an International Journal for Oncology Research·B M BurtY Fong
Nov 10, 2004·Genomics·B Altenberg, K O Greulich
Mar 30, 2010·Biochemical and Biophysical Research Communications·Catherine L Neary, John G Pastorino
Nov 25, 2016·Journal of Cellular Biochemistry·Baoxing JiaYahui Liu
Nov 26, 2016·Best Practice & Research. Clinical Obstetrics & Gynaecology·Neville F Hacker, Archana Rao
Dec 21, 2016·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M K WilsonUNKNOWN participants of the Fifth Ovarian Cancer Consensus Conference
Dec 9, 2017·Cell Research·Paolo Ettore PorporatoLorenzo Galluzzi
Dec 13, 2017·Annals of Oncology : Official Journal of the European Society for Medical Oncology·E A Eisenhauer
Apr 10, 2018·Obstetrics and Gynecology·Usha MenonAleksandra Gentry-Maharaj
Jun 19, 2018·Expert Review of Anticancer Therapy·Kathleen G Essel, Kathleen N Moore
Jun 28, 2018·Advances in Experimental Medicine and Biology·Sminu Bose, Anne Le
Jul 1, 2018·Biochimica Et Biophysica Acta. Reviews on Cancer·Linchong SunPing Gao
Oct 10, 2018·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·H G VuongT Kondo
Nov 6, 2018·Hematology/oncology Clinics of North America·Brian Orr, Robert P Edwards
Nov 27, 2018·Journal of Cellular Biochemistry·Yitong ZhouLudong Tan
Jan 9, 2019·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 23, 2019·Critical Reviews in Biochemistry and Molecular Biology·Shanmugasundaram Ganapathy-Kanniappan
Feb 15, 2019·CA: a Cancer Journal for Clinicians·Carol E DeSantisRebecca L Siegel
Mar 15, 2019·International Journal of Molecular Sciences·Marianne T BjerreKarina D Sørensen
Mar 25, 2019·European Journal of Nuclear Medicine and Molecular Imaging·Yoshiaki AbeKosei Matsue
Aug 23, 2019·Cellular and Molecular Life Sciences : CMLS·Jinshou YangYupei Zhao
Oct 30, 2019·World Journal of Gastroenterology : WJG·Li-Li ZhouYan-Qiu Song
Dec 13, 2019·Nature·Caroline R AmendolaMark R Philips

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Methods Mentioned

BETA
Transfection
Fluorescence
PCR
Electrophoresis
light microscopy
ELISA

Software Mentioned

GSEA
Ggplot2
Gene Set Enrichment Analysis ( GSEA )
GSEA Enrichment

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