Prognostic significance of Cytokeratin 20-positive lymph node vascular endothelial growth factor A mRNA and chromodomain helicase DNA binding protein 4 in pN0 colorectal cancer patients

Oncotarget
Sze Chuen Cesar WongJohn Kwok Cheung Chan

Abstract

Cytokeratin 20-positive cells in lymph nodes from pN0 colorectal cancer (CRC) patients were detected previously by us. The aims of this study were to investigate which tumor metastasis-related genes were involved and their potential clinical significance. Fourteen of 84 (17%) genes were differentially expressed by at least 2-fold. Among them, 10 genes were up-regulated whereas 4 genes were down-regulated. Those differential expressed genes were validated in the second cohort of specimens. Follow-up analysis for 60 months showed that patients with lymph nodevascular endothelial growth factor A (VEGF-A)mRNA andchromodomain helicase DNA binding protein 4 (CHD4)mRNA expression higher than the median copies had significantly shorter time to recurrence than those with lower than the median copies. Multivariate analysis showed thatVEGF-AmRNA,CHD4mRNA and lymphatic vessel involvement were independent prognostic factors for disease recurrence. VEGF-A mRNA andCHD4mRNA were up-regulated in CK20-positive pN0 lymph nodes and they may have prognostic significance in pN0 CRC patients. Two cohorts of lymph node specimens from pN0 CRC patients of each with and without CK20-positive cells were recruited. In the first cohort, tumor metastasis gen...Continue Reading

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Methods Mentioned

BETA
PCR

Software Mentioned

Statistical Package for the Social Sciences
GraphPad
SPSS
GraphPad Prism

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