PMID: 9179053Jun 1, 1997Paper

Prognostic significance of DNA ploidy and proliferation in 309 colorectal carcinomas as determined by two-color multiparametric DNA flow cytometry

Cancer
R J ZarboP Mackowiak

Abstract

Although DNA flow cytometry has been shown to be of independent value in determining the prognosis of colorectal carcinoma, a number of well-designed studies with contradictory findings have left unresolved the clinical significance of DNA ploidy and proliferation in biologically meaningful subsets of colorectal carcinoma cases. To evaluate the prognostic significance of DNA ploidy and proliferation as determined by flow cytometry in a prospective series of 309 human colorectal carcinomas with 4-6 years of follow-up, fresh tumors were mechanically dissociated into whole cell suspensions and dual fluorescence-labeled to allow gated analysis of subpopulations with phenotypic markers. Software programs with histogram-dependent algorithms employing background, aggregate, and debris correction were used in DNA and cell cycle quantitation. Data were analyzed according to recommendations of the 1992 DNA Flow Cytometry Consensus Conference. None of the clinical, site, or staging parameters, including TNM stage variables, correlated with any flow cytometric DNA ploidy or proliferation measurement. Tumors classified as DNA aneuploid or tetraploid, by any definition, did not differ in prognosis or correlate with stage or any pathologic pa...Continue Reading

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Citations

Sep 25, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Johan BondiHåvard Danielsen
Oct 19, 2005·International Journal of Cancer. Journal International Du Cancer·Shih-Ching ChangChin-Wen Chi
Nov 26, 2015·Nature Reviews. Clinical Oncology·Håvard E DanielsenMarco Novelli
Mar 31, 2005·Cancer Letters·Elisenda VendrellMiguel A Peinado
Jun 10, 2015·International Journal of Colorectal Disease·Tilman LaubertUNKNOWN North German Tumorbank of Colorectal Cancer (ColoNet) consortium
Jul 15, 1999·Journal of Surgical Oncology·J S Spratt, J S Meyer
Nov 24, 1999·International Journal of Cancer. Journal International Du Cancer·S BuglioniM Mottolese
Mar 29, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Carmen J AllegraRichard M Goldberg
Jul 13, 2002·Expert Review of Anticancer Therapy·F GrazianoS Cascinu
Nov 5, 2003·Annals of Surgical Oncology·Jen-Kou LinAnna Fen-Yau Li
Sep 18, 2007·Diseases of the Colon and Rectum·Sergio E A AraujoIvan Cecconello

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