Prognostic significance of flow cytometry deoxyribonucleic acid analysis of human oligodendrogliomas
Flow cytometry was used to determine the deoxyribonucleic acid ploidy and proliferative activity of 60 oligodendrogliomas and oligoastrocytomas. The relationships among survival, ploidy, proliferation, histological features, and clinical variables were analyzed. Survival was strongly associated with the S-phase fraction (P < 0.001). Three groups with significantly different survival rates were defined, based on S-phase fraction ranges of < 3%, 3 to 5.9%, and > 6%. Significant associations between survival and age at diagnosis (P < 0.001), tumor grade (P < 0.001), and extent of surgery (P < 0.01) were found also. The poor correlation between mitotic figures and survival in oligodendrogliomas that has been reported previously emphasizes the need for an accurate method to measure proliferative activity. Our study demonstrated the usefulness of the flow cytometry-determined S-phase fraction in this regard and demonstrated that specific reference ranges could be defined for clinical application. In contrast, the determination of ploidy by flow cytometry was not useful in the evaluation of oligodendrogliomas.
Prognostic implications of glial fibrillary acidic protein containing cell types in oligodendrogliomas
Flow-cytometric DNA analysis and immunohistochemical measurement of Ki-67 and BUdR labeling indices in human brain tumors
Cytologic composition of the untreated glioblastoma with implications for evaluation of needle biopsies
Immunophenotypic differences between normal glia, astrocytomas and malignant gliomas: correlations with karyotype, natural history and survival
Flow cytometric determination of modal DNA population in relation to proliferative potential of human intracranial neoplasms
Application of DNA flow cytometry to paraffin-embedded archival material for the study of aneuploidy and its clinical significance
Regional heterogeneity in the proliferative activity of human gliomas as measured by the Ki-67 labeling index
Improving diagnostic accuracy and interobserver concordance in the classification and grading of primary gliomas
DNA ploidy and cell-cycle analysis in intracranial meningiomas and hemangiopericytomas: a study with high-resolution DNA flow cytometry
Nuclear multiparameter flow cytometric DNA analysis of human brain tumors: correlation of DNA content with tumor histology and clinical behavior
Prognostic factors in low grade (WHO grade II) gliomas of the cerebral hemispheres: the role of surgery
Age and bromodeoxyuridine labelling index as prognostic factors in high-grade gliomas treated with surgery and radiotherapy
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