Prognostic Significance of Promoter DNA Hypermethylation of cysteine dioxygenase 1 (CDO1) Gene in Primary Breast Cancer.

PloS One
Naoko MinataniMasahiko Watanabe

Abstract

Using pharmacological unmasking microarray, we identified promoter DNA methylation of cysteine dioxygenase 1 (CDO1) gene in human cancer. In this study, we assessed the clinicopathological significance of CDO1 methylation in primary breast cancer (BC) with no prior chemotherapy. The CDO1 DNA methylation was quantified by TaqMan methylation specific PCR (Q-MSP) in 7 BC cell lines and 172 primary BC patients with no prior chemotherapy. Promoter DNA of the CDO1 gene was hypermethylated in 6 BC cell lines except SK-BR3, and CDO1 gene expression was all silenced at mRNA level in the 7 BC cell lines. Quantification of CDO1 methylation was developed using Q-MSP, and assessed in primary BC. Among the clinicopathologic factors, CDO1 methylation level was not statistically significantly associated with any prognostic factors. The log-rank plot analysis elucidated that the higher methylation the tumors harbored, the poorer prognosis the patients exhibited. Using the median value of 58.0 as a cut-off one, disease specific survival in BC patients with CDO1 hypermethylation showed significantly poorer prognosis than those with hypomethylation (p = 0.004). Multivariate Cox proportional hazards model identified that CDO1 hypermethylation was p...Continue Reading

References

Aug 28, 1998·Science·D R Green, J C Reed
Nov 24, 1999·Free Radical Biology & Medicine·O W Griffith
Aug 30, 2000·Nature·C M PerouD Botstein
Nov 28, 2006·Biochemical and Biophysical Research Communications·Derek B Oien, Jackob Moskovitz
Dec 13, 2006·Genes & Development·Angela H TingStephen B Baylin
Feb 27, 2007·Cell·Peter A Jones, Stephen B Baylin
Nov 21, 2007·Chemical Communications : Chem Comm·Crisjoe A Joseph, Michael J Maroney
Sep 10, 2008·Mutation Research·Suhasni GopalakrishnanKeith D Robertson
Nov 18, 2008·Amino Acids·M H StipanukL L Hirschberger
Feb 11, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Joel S ParkerPhilip S Bernard
Sep 25, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S J DawsonC Caldas
Jun 29, 2011·Annals of Oncology : Official Journal of the European Society for Medical Oncology·A GoldhirschUNKNOWN Panel members
Sep 10, 2011·Frontiers in Pharmacology·Paolo E PorporatoPierre Sonveaux
May 1, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jana JeschkeNita Ahuja
Dec 20, 2013·Cancer Research·Antony PrabhuPrakash Chinnaiyan
Sep 19, 2014·World Journal of Gastrointestinal Oncology·Ambedkar Raj Kulandai VeluIlavarasi Sinduja
Dec 4, 2014·PloS One·Keishi YamashitaMasahiko Watanabe

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Citations

Mar 31, 2017·International Journal of Molecular Sciences·Keigo YokoiMasahiko Watanabe
Sep 29, 2018·Cancer Science·Keishi YamashitaMasahiko Watanabe
Feb 9, 2017·Oncotarget·Esperanza Martín-SánchezDavid Guerrero-Setas
Apr 26, 2017·International Journal of Experimental Pathology·Bibekananda SarkarAnil K Mantha
Feb 12, 2017·Diseases of the Esophagus : Official Journal of the International Society for Diseases of the Esophagus·K KojimaM Watanabe
Dec 13, 2018·Annals of Surgical Oncology·Sudeep Banerjee, Jason K Sicklick
Jan 21, 2020·BioMed Research International·Rui XuJukun Song
Jun 30, 2016·PloS One·Rubiceli Medina-AguilarCésar López-Camarillo

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Methods Mentioned

BETA
surgical resection
PCR
reverse transcribed PCR

Software Mentioned

SAS

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