Prognostic significance of TP53 accumulation in human primary breast cancer: comparison between a rapid quantitative immunoassay and SSCP analysis

International Journal of Cancer. Journal International Du Cancer
H H De WitteE M Berns

Abstract

TP53 accumulation in human primary breast carcinomas was studied by a quantitative luminometric immunoassay (LIA), and TP53 gene alterations, exons 5-8, were examined by single-strand conformation polymorphism (SSCP) analysis. In 48 of 142 breast tumor samples, a TP53 gene alteration was identified. In tumor samples without a TP53 gene alteration, the median cytosolic TP53 protein level, as determined by LIA, was 0.4 ng/mg protein (range 0-70.8 ng/mg protein), whereas the median TP53 protein level for tumor samples with a TP53 gene alteration was 10 times higher, i.e., 4.1 ng/mg protein (range 0.1-176.0 ng/mg protein). Despite a significant correlation between the outcome of LIA and SSCP, a disagreement was found in 22% of cases analyzed. Significant correlations were found between TP53 protein accumulation and low estrogen receptor content, and with a shorter relapse-free as well as overall survival, with a median duration of follow-up of 100 months. Due to its rapid and easy performance on routinely prepared cytosols, the LIA for TP53 protein may be useful in evaluating the prognostic impact of TP53 protein accumulation in human primary breast cancer.

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Citations

Mar 6, 2003·Human Mutation·Anne-Lise Børresen-Dale
Jul 17, 2001·Journal of Surgical Oncology·W FuS C Young
Mar 17, 2000·International Journal of Cancer. Journal International Du Cancer·H BlaszykS S Sommer
Jul 26, 2006·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Gregor WesthofWolfgang Hatzmann
Jun 24, 2008·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Li TalleyA R Frost
Mar 5, 1999·Breast Cancer Research and Treatment·R M Elledge, D C Allred
May 16, 1998·International Journal of Cancer. Journal International Du Cancer·M A LevesqueE P Diamandis
Feb 23, 2010·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Aneta Janiec-JankowskaUrszula Najmoła
Jun 15, 1997·Analytical Chemistry·D J AndersonK A Davis

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