Prognostic value of mature microRNA-21 and microRNA-205 overexpression in non-small cell lung cancer by quantitative real-time RT-PCR

Clinical Chemistry
Athina MarkouEvi S Lianidou

Abstract

microRNA (miRNA) expression profiles are being intensively investigated for their involvement in carcinogenesis. We evaluated the prognostic value of mature microRNA-21 (miR-21) and mature microRNA-205 (miR-205) overexpression in non-small cell lung cancer (NSCLC). We studied 48 pairs of NSCLC fresh frozen tissue specimens collected at time of surgery and before chemotherapy. Highly specific amplification and quantification of mature miR-21 and mature miR-205 was achieved using looped real time RT-PCR. miRNA expression, determined by real time RT-PCR, was defined by DeltaDeltaCt measurements. We detected overexpression of mature miR-21 in 25 (52.0%) of the 48 NSCLC paired specimens and overexpression of miR-205 in 31 (64.6%). Overexpression was assessed after comparison of miRNA expression in NSCLC tissues and in their corresponding noncancerous tissues with respect to U6 expression. During the follow-up period, 29 of 48 (60.4%) patients relapsed, and 23 of 48 died (47.9%). Mature miR-21 was upregulated in 16 of 29 (55.2%) patients who relapsed and 15 of 23 (65.2%) patients who died. Mature miR-205 was overexpressed in 19 of 29 patients who relapsed (65.5%) and 15 of 23 patients who died (65.2%). Mature miR-21 overexpression co...Continue Reading

References

Feb 20, 1998·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·S RamalingamG P Kalemkerian
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 20, 2004·Proceedings of the National Academy of Sciences of the United States of America·George Adrian CalinCarlo M Croce
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Chang-Gong LiuCarlo M Croce
Mar 23, 2005·Nature Methods·Peter T NelsonZissimos Mourelatos
May 4, 2005·Cancer Research·Richard I Gregory, Ramin Shiekhattar
Jun 10, 2005·The New England Journal of Medicine·Matthias Eder, Michaela Scherr
Jun 10, 2005·Nature·Jun LuTodd R Golub
Jul 19, 2005·Cancer Research·Jennifer A ChanKenneth S Kosik
Jul 26, 2005·Biochemical and Biophysical Research Communications·S A CiafrèM G Farace
Aug 17, 2005·Cancer Research·Marilena V IorioCarlo M Croce
Sep 30, 2005·Nucleic Acids Research·Jinmai JiangThomas D Schmittgen
Nov 30, 2005·Nucleic Acids Research·Caifu ChenKarl J Guegler
Dec 20, 2005·Current Opinion in Genetics & Development·Scott M Hammond
Jan 26, 2006·Nucleic Acids Research·Fuchou TangM Azim Surani
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano VoliniaCarlo M Croce
Feb 24, 2006·British Journal of Cancer·H-W Hwang, J T Mendell
Mar 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jordan M CumminsVictor E Velculescu
Mar 15, 2006·Cancer Cell·Nozomu YanaiharaCurtis C Harris
Mar 25, 2006·Nature Reviews. Cancer·Aurora Esquela-Kerscher, Frank J Slack
Sep 16, 2006·Nucleic Acids Research·Shuji TakadaHiroyuki Mano
Sep 23, 2006·Developmental Biology·Baohong ZhangTodd A Anderson
Oct 31, 2006·Oncogene·M-L SiY-Y Mo
Mar 17, 2007·The Journal of Biological Chemistry·Shuomin ZhuYin-Yuan Mo
Sep 11, 2007·Urologic Oncology·Fedra GottardoRaffaele Baffa
Nov 10, 2007·The Journal of Biological Chemistry·Lisa B FrankelAnders H Lund

❮ Previous
Next ❯

Citations

Mar 30, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hui ZhouBing-Xiu Xiao
Nov 7, 2012·Surgery Today·Isamu Hoshino, Hisahiro Matsubara
Oct 19, 2011·Cancer Metastasis Reviews·Ashleigh E MooreDan A Dixon
Nov 12, 2010·Clinical & Experimental Metastasis·Boye Schnack NielsenHans Jørgen Nielsen
Apr 25, 2012·Frontiers of Medicine·Ji Qi, David Mu
Apr 6, 2013·Cell Biochemistry and Biophysics·Amal A Abd-El-FattahMariam Lotfy Aboulftouh
Jan 2, 2013·Medical Oncology·Yong HuangXiang Chao Cheng
Jun 18, 2011·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·María Cortés-Sempere, Inmaculada Ibáñez de Cáceres
Nov 2, 2011·The Breast : Official Journal of the European Society of Mastology·Marilena V IorioElda Tagliabue
Mar 23, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Jing WenMing Guo
Jan 25, 2013·Nature Reviews. Cancer·Bram De Craene, Geert Berx
Nov 4, 2010·Nature Reviews. Clinical Oncology·Nicole M A WhiteGeorge M Yousef
Feb 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mattia BoeriGabriella Sozzi
Feb 8, 2013·Genetic Testing and Molecular Biomarkers·Yongchun ShenFuqiang Wen
Dec 4, 2009·Carcinogenesis·Aaron J SchetterCurtis C Harris
Jul 3, 2013·Japanese Journal of Clinical Oncology·Yan WangMing Chu
Jun 1, 2012·The Cancer Journal·Aaron J SchetterCurtis C Harris
Nov 4, 2010·Respirology : Official Journal of the Asian Pacific Society of Respirology·Krishna Bajee SriramKwun Meng Fong
Jan 14, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Maria Teresa LandiEna Wang
Feb 26, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Motonobu SaitoCurtis C Harris
May 23, 2012·Journal of Translational Medicine·Jiri SanaOndrej Slaby
Jun 20, 2013·Radiation Oncology·Xiao-chun WangJian-Chao Luo
Jun 11, 2010·BMC Systems Biology·Qinghua JiangYadong Wang
Feb 10, 2011·Journal of Experimental & Clinical Cancer Research : CR·Wei-Sheng ChenZhi-Gang She
Apr 19, 2013·Journal of Clinical Bioinformatics·Lei DongJia Yu
Nov 4, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Marilena V Iorio, Carlo M Croce
Jan 28, 2010·Journal of Cell Science·Stephanie B GreeneJeffrey M Rosen
Jul 7, 2011·PloS One·Jih-Hsiang LeeGiuseppe Giaccone
Jan 22, 2013·PloS One·Michelle LenarduzziFei-Fei Liu
Jul 20, 2011·Clinical Chemistry and Laboratory Medicine : CCLM·Athina MarkouEvi Lianidou
Sep 17, 2011·Future Oncology·Irene Cherni, Glen J Weiss

❮ Previous
Next ❯

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