Prognostic value of protein inhibitor of activated STAT3 in breast cancer patients receiving hormone therapy

BMC Cancer
Sheau-Fang YangYao-Tsung Yeh

Abstract

Deregulated signal transducer and activator of transcription 3 (STAT3) signaling has been well documented in certain cancers. Alterations in specific negative regulators, such as protein inhibitor of activated STAT3 (PIAS3), may contribute to cancer development. The expression of total PIAS3 was determined in 100 paired cancerous and non-cancerous breast tissues by immunoblotting and was statistically analyzed along with the clinicopathological characteristics and overall survival of the patients. XTT, immunoblotting, and chromatin immunoprecipitation (Chip) were used to examine the biological effect of PIAS3 in breast cancer cells. Hormone therapy failed to improve the overall survival in patients presenting with increased PIAS3 expression. Ectopic PIAS3 overexpression increased the proliferation and expression of cyclin D1 in estrogen receptor (ER)-positive MCF-7 and T47D cells, but decreased those in ER-negative MDA-MB-231 and SKBR3 cells. Furthermore, PIAS3 overexpression attenuated cytotoxicity of tamoxifen and increased proliferation and cyclin D1 expression in MCF-7 cells. PIAS3 also decreased the binding of itself on the cyclin D1 promoter and this decreased binding was not affected by tamoxifen. PIAS3 may serve as a bi...Continue Reading

References

Dec 31, 1997·Science·C D ChungK Shuai
May 5, 1998·Molecular and Cellular Biology·J F BrombergJ E Darnell
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·M SabbahG Redeuilh
Dec 26, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Qian ZhangMariusz A Wasik
May 3, 2002·The Journal of Biological Chemistry·Meng-Min LiuPeter J Kushner
Jun 18, 2005·Molecular Endocrinology·Stephanie SentisLaura Corbo
Jun 2, 2006·The British Journal of Surgery·S-N WangK-T Lee
Oct 13, 2006·Neoplasia : an International Journal for Oncology Research·Yoshitaka OgataIchiro Kawase
Feb 22, 2011·Clinics and Research in Hepatology and Gastroenterology·Liang-ming LiuJi-xiang Zhang
Apr 19, 2011·Molecular Oncology·Amy KlugeAfshin Dowlati
Dec 6, 2011·BMC Cancer·Wen-Yong WuXiang-Ling Meng
Dec 6, 2011·PloS One·Janeen AzareJacqueline Bromberg
Mar 1, 2012·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Amir SonnenblickTamar Peretz
Aug 16, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Snehal DabirAfshin Dowlati

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Methods Mentioned

BETA
sumoylation
protein assay
electrophoresis
PCR
immunoprecipitation

Software Mentioned

Image J
SPSS

Related Concepts