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Programmable CRISPR interference for gene silencing using Cas13a in mosquitoes

bioRxiv

Nov 18, 2019

Aditi KulkarniJian-Nong Xu

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Abstract

In the CRISPR-Cas systems, Cas13a is an RNA-guided RNA nuclease specifically targeting single strand RNA. In this study we developed a Cas13a mediated CRISPR interference tool to target mRNA for gene silencing in mosquitoes and tested the machinery in two mosquito species. A Cas13a expr...read more

Mentioned in this Paper

Blood
Genes
Insecta
Culicidae
Plasmids
RNA
RNA, Double-Stranded
RNA, Messenger
Vitellogenins
Primitive Midgut Structure
10
3
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Programmable CRISPR interference for gene silencing using Cas13a in mosquitoes

bioRxiv

Nov 18, 2019

Aditi KulkarniJian-Nong Xu

DOI: 10.1101/846055

Abstract

In the CRISPR-Cas systems, Cas13a is an RNA-guided RNA nuclease specifically targeting single strand RNA. In this study we developed a Cas13a mediated CRISPR interference tool to target mRNA for gene silencing in mosquitoes and tested the machinery in two mosquito species. A Cas13a expr...read more

Mentioned in this Paper

Blood
Genes
Insecta
Culicidae
Plasmids
10
3

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