Programmable delivery of DNA through a nanopipet

Analytical Chemistry
Liming YingDavid Klenerman

Abstract

We report the pulsed delivery of single-stranded DNA molecules through a nanopipet. The conical geometry of the pipet leads to a localized electric field, since all of the potential drop occurs in the tip region. Pulsatile delivery of DNA molecules can be achieved in an experimentally simple way with high precision by controlling the applied voltage. Single-molecule detection and fluorescence correlation spectroscopy in the nanopipet enable us to determine the number of molecules delivered. Anomalous slow diffusion of the DNA molecules in the pipet has also been observed. This nanopumping technique may have potential applications in local drug delivery and nanofabrication of biomolecules on surfaces in aqueous environments.

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Citations

Mar 15, 2008·Analytical and Bioanalytical Chemistry·Nicholas DouvilleShuichi Takayama
Dec 26, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·David KlenermanSimon J Davis
Jan 1, 2010·Sensors·Kuo-Kang LiuFan-Gang Tseng
Dec 4, 2012·Sensors·Patrick HappelIrmgard D Dietzel
Aug 25, 2007·Nanomedicine·David Klenerman, Yuri Korchev
Apr 25, 2012·Annual Review of Analytical Chemistry·Chiao-Chen ChenLane A Baker
Jul 12, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Minkyung KangPatrick R Unwin
Aug 2, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Georg RempferJoost de Graaf
Aug 15, 2014·The Analyst·Wenqing ShiLane A Baker
Oct 20, 2006·Angewandte Chemie·Samuel S WhiteLiming Ying
Nov 27, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hitoshi Shiku
May 21, 2010·Physical Chemistry Chemical Physics : PCCP·Yasufumi TakahashiTomokazu Matsue
Jun 22, 2010·The Analyst·Celeste A MorrisLane A Baker
Sep 29, 2005·Physical Chemistry Chemical Physics : PCCP·Liming YingDavid Klenerman
Oct 27, 2005·Angewandte Chemie·Kit T RodolfaDavid Klenerman
Aug 15, 2013·The Analyst·Rahul ThakarLane A Baker
Jul 21, 2009·Biochemical Society Transactions·Liming Ying
Feb 23, 2010·The Analyst·Li-Qun Gu, Ji Wook Shim
Jan 1, 2014·Nano Convergence·Tomohide TakamiTomoji Kawai
Oct 21, 2004·Biochemical Society Transactions·H LiD Klenerman
May 10, 2017·Proceedings. Mathematical, Physical, and Engineering Sciences·Ashley PagePatrick R Unwin
Apr 13, 2006·Nano Letters·Mordechai Sokuler, Levi A Gheber
Oct 11, 2017·Analytical Chemistry·David PerryPatrick R Unwin
Mar 22, 2015·ACS Nano·Aleksandar P IvanovJoshua B Edel
Dec 6, 2012·Journal of the American Chemical Society·Richard W ClarkeDavid Klenerman
Aug 31, 2017·Analytical Chemistry·Hai-Ling LiuKang Wang
Oct 23, 2012·Analytical Chemistry·Matthew CaldwellGuy W J Moss
Jan 21, 2017·Analytical Chemistry·Yasufumi TakahashiTomokazu Matsue

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