Programmable flow system for automation of oxygen radical absorbance capacity assay using pyrogallol red for estimation of antioxidant reactivity

Talanta
Inês I RamosMarcela A Segundo

Abstract

An automated oxygen radical absorbance capacity (ORAC) method based on programmable flow injection analysis was developed for the assessment of antioxidant reactivity. The method relies on real time spectrophotometric monitoring (540 nm) of pyrogallol red (PGR) bleaching mediated by peroxyl radicals in the presence of antioxidant compounds within the first minute of reaction, providing information about their initial reactivity against this type of radicals. The ORAC-PGR assay under programmable flow format affords a strict control of reaction conditions namely reagent mixing, temperature and reaction timing, which are critical parameters for in situ generation of peroxyl radical from 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH). The influence of reagent concentrations and programmable flow conditions on reaction development was studied, with application of 37.5 µM of PGR and 125 mM of AAPH in the flow cell, guaranteeing first order kinetics towards peroxyl radicals and pseudo-zero order towards PGR. Peroxyl-scavenging reactivity of antioxidants, bioactive compounds and phenolic-rich beverages was estimated employing the proposed methodology. Recovery assays using synthetic saliva provided values of 90 ± 5% for reduced ...Continue Reading

References

Oct 1, 1986·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·M TambaM Quintiliani
Jan 1, 1995·Critical Reviews in Food Science and Nutrition·B HalliwellO I Aruoma
Mar 1, 1993·Free Radical Biology & Medicine·G CaoR G Cutler
Sep 6, 2003·International Journal of Cardiology·Ferdinando FranzoniFabio Galetta
Sep 19, 2006·The International Journal of Biochemistry & Cell Biology·Marian ValkoJoshua Telser
Oct 13, 2006·Journal of Agricultural and Food Chemistry·Jing Jing GaoBo Tang
Apr 1, 2008·Analytica Chimica Acta·Luís M MagalhãesJosé L F C Lima
Apr 9, 2008·Journal of Agricultural and Food Chemistry·Yo OmataEtsuo Niki
Mar 12, 2009·The Journal of Organic Chemistry·Malgorzata MusialikGrzegorz Litwinienko
Jun 22, 2010·Analytical and Bioanalytical Chemistry·Joana P N RibeiroJosé L F C Lima
Jul 20, 2010·Chemico-biological Interactions·Dominique ZiechMihalis I Panayiotidis
Sep 30, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Max RomeroCamilo López-Alarcón
Feb 11, 2011·Luminescence : the Journal of Biological and Chemical Luminescence·André R T S AraujoVíctor Cerdà
May 12, 2011·Journal of Pharmaceutical Sciences·Jay WerberJunyan A Ji
Nov 22, 2011·Archives of Toxicology·İlhami Gülçin
Apr 11, 2012·BioFactors·Mizuki TakashimaEtsuo Niki
Jan 24, 2013·Analytica Chimica Acta·Camilo López-Alarcón, Ana Denicola
May 23, 2014·Diabetes/metabolism Research and Reviews·Randhir SinghRakesh Gollen
Aug 19, 2014·Journal of Atherosclerosis and Thrombosis·Soo Lim, Philip Barter
Jun 22, 2015·Journal of Pharmaceutical and Biomedical Analysis·Sara S MarquesMarcela A Segundo

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.