Programmable polyproteams built using twin peptide superglues

Proceedings of the National Academy of Sciences of the United States of America
Gianluca VeggianiMark Howarth

Abstract

Programmed connection of amino acids or nucleotides into chains introduced a revolution in control of biological function. Reacting proteins together is more complex because of the number of reactive groups and delicate stability. Here we achieved sequence-programmed irreversible connection of protein units, forming polyprotein teams by sequential amidation and transamidation. SpyTag peptide is engineered to spontaneously form an isopeptide bond with SpyCatcher protein. By engineering the adhesin RrgA from Streptococcus pneumoniae, we developed the peptide SnoopTag, which formed a spontaneous isopeptide bond to its protein partner SnoopCatcher with >99% yield and no cross-reaction to SpyTag/SpyCatcher. Solid-phase attachment followed by sequential SpyTag or SnoopTag reaction between building-blocks enabled iterative extension. Linear, branched, and combinatorial polyproteins were synthesized, identifying optimal combinations of ligands against death receptors and growth factor receptors for cancer cell death signal activation. This simple and modular route to programmable "polyproteams" should enable exploration of a new area of biological space.

References

Dec 20, 1994·Proceedings of the National Academy of Sciences of the United States of America·T K ChangJ A Wells
Jun 10, 2003·The Journal of Biological Chemistry·Patrick G Telmer, Brian H Shilton
Aug 5, 1965·Journal of the American Chemical Society·R L LETSINGER, V MAHADEVAN
Oct 14, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Garland R Marshall
Oct 17, 2003·Nature·Won-Ki HuhErin K O'Shea
Jun 23, 2004·Protein Engineering, Design & Selection : PEDS·M WikmanS Ståhl
Jul 3, 2004·Clinical Chemistry·John RussellStephen Stroupe
Nov 6, 2004·Nature Biotechnology·François Baneyx, Mirna Mujacic
Mar 25, 2006·Angewandte Chemie·Laura L KiesslingLaura E Strong
Jun 6, 2006·Journal of Molecular Biology·Christofer LendelTorleif Härd
Apr 5, 2007·Nature Protocols·Helena Hernández, Carol V Robinson
Feb 26, 2009·Cell·Hani S Zaher, Rachel Green
Jan 22, 2010·Biotechnology and Applied Biochemistry·Jingjing LiTorbjörn Gräslund
Mar 20, 2010·Journal of the American Chemical Society·Bijan Zakeri, Mark Howarth
Jun 11, 2010·Applied Microbiology and Biotechnology·Iris H WalkerPaul D Riggs
Aug 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Charles EigenbrotTorleif Härd
Oct 16, 2010·Nature Reviews. Immunology·Martin F Bachmann, Gary T Jennings
Nov 3, 2010·Journal of Molecular Recognition : JMR·Paolo PengoGiorgio Fassina
Nov 9, 2010·Trends in Biochemical Sciences·Hae Joo Kang, Edward N Baker
May 4, 2011·Angewandte Chemie·Maximilian Wei-Lin Popp, Hidde L Ploegh
Jun 28, 2011·Science·Camille J DelebecqueFaisal A Aldaye
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bijan ZakeriMark Howarth
Apr 20, 2012·Chembiochem : a European Journal of Chemical Biology·Pijus K SasmalEric Meggers
Jun 29, 2012·Trends in Pharmacological Sciences·Edmund A RossiChien-Hsing Chang
Jul 4, 2012·The Journal of Biological Chemistry·Patricia Carvajal-VallejosStefan R Schmidt
Oct 11, 2012·Immunological Reviews·Omer DushekP Anton van der Merwe
Oct 17, 2012·Chembiochem : a European Journal of Chemical Biology·Justin A ModicaMilan Mrksich
Feb 6, 2013·Nuclear Medicine and Biology·S M van den BoschM S Robillard
Apr 13, 2013·Science·Jordan M FletcherDerek N Woolfson
Apr 30, 2013·Nature Chemical Biology·Helena GradišarRoman Jerala
Jul 11, 2013·Bioconjugate Chemistry·Mohammad RashidianMark D Distefano
Aug 24, 2013·Journal of the American Chemical Society·Wen-Bin ZhangFrances H Arnold
Nov 28, 2013·Angewandte Chemie·Ron M VersteegenMarc S Robillard
Jan 15, 2014·Cytokine & Growth Factor Reviews·Pamela M Holland
Jan 21, 2014·ACS Chemical Biology·David M PattersonJennifer A Prescher
Feb 1, 2014·Angewandte Chemie·Andrew T KruegerBarbara Imperiali
Mar 19, 2014·Chemical Science·Neel H Shah, Tom W Muir
Mar 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jacob O FiererMark Howarth
Mar 25, 2014·Nature Materials·Allen Y ChenTimothy K Lu
May 2, 2014·Nature Methods·Sriram Kosuri, George M Church

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Citations

Jul 16, 2016·Microbial Biotechnology·Claudia Schmidt-Dannert, Fernando Lopez-Gallego
Apr 4, 2017·Chembiochem : a European Journal of Chemical Biology·Arnaud Gautier, Raphaël Rodriguez
Apr 5, 2017·Organic & Biomolecular Chemistry·M B QuinC Schmidt-Dannert
Jan 14, 2017·Chemical Communications : Chem Comm·P G YoungC J Squire
Jun 28, 2017·PloS One·Marlene PröschelHeinrich Sticht
Dec 12, 2017·Protein Engineering, Design & Selection : PEDS·M HinrichsenL Regan
Feb 15, 2018·Applied and Environmental Microbiology·H Bart van den Berg van SaparoeaJoen Luirink
Oct 11, 2017·Chemical Science·Yajie LiuWen-Bin Zhang
May 18, 2017·Chembiochem : a European Journal of Chemical Biology·Veronica PessinoBo Huang
Oct 13, 2017·Angewandte Chemie·Anthony H KeebleMark Howarth
Oct 26, 2018·Chembiochem : a European Journal of Chemical Biology·Ashley R SutherlandC Ronald Geyer
Feb 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mariana I NevesCristina C Barrias
Dec 31, 2019·Biotechnology and Bioengineering·Francesca ManeaCaroline M Ajo-Franklin
Apr 28, 2016·Journal of Nanobiotechnology·Susan ThraneAdam F Sander
Mar 11, 2020·Annual Review of Chemical and Biomolecular Engineering·Emily A BerckmanWilfred Chen
Aug 26, 2020·Chemical Communications : Chem Comm·Emily A Berckman, Wilfred Chen
May 24, 2017·Proceedings of the National Academy of Sciences of the United States of America·Ri WangFei Sun
Mar 7, 2019·Frontiers in Plant Science·Juliane RöderUlrich Commandeur
Jun 9, 2020·Frontiers in Molecular Biosciences·Byeongseon YangMichael A Nash
Sep 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Vittoria ChimissoCornelia G Palivan
May 6, 2019·International Journal of Molecular Sciences·Daniel HatlemJack C Leo
Mar 14, 2018·Advanced Functional Materials·Christopher M Madl, Sarah C Heilshorn
Apr 22, 2020·ELife·Paul J Wichgers SchreurJeroen Kortekaas
Mar 3, 2020·Frontiers in Bioengineering and Biotechnology·Jin Xiang WongBernd H A Rehm
Aug 9, 2020·Scientific Reports·Florian BourdeauxMartin Grininger
Jun 2, 2020·Frontiers in Microbiology·H Bart van den Berg van SaparoeaWouter S P Jong
Apr 18, 2018·Nature Communications·Wenwei MaEnrico Ferrari
Aug 29, 2018·Proceedings of the National Academy of Sciences of the United States of America·Jaime Andrés Rivas-PardoJulio M Fernández
Sep 12, 2020·International Journal of Molecular Sciences·Tim HofmannAchim Doerner
Dec 12, 2019·Proceedings of the National Academy of Sciences of the United States of America·Anthony H KeebleMark Howarth

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