Programmed cell death and Bcl-2 protection in very low oxygen

Nature
M D Jacobson, M C Raff

Abstract

Programmed cell death (PCD) is a fundamental feature of animal cells, but the mechanism remains unknown. Similarly, the Bcl-2 oncoprotein can suppress PCD in a variety of cell types and circumstances, but it is not known how it does so. It has been suggested that PCD involves the generation of reactive oxygen species (ROS) and that Bcl-2 protects against PCD by inhibiting the generation or action of ROS. To determine whether ROS are required for PCD, we cultured cells in a near-anaerobic atmosphere where the generation of ROS would be expected not to occur, or at least to be greatly reduced. We find that these conditions inhibit PCD induced by ROS-generating agents but do not inhibit PCD induced by other means. Furthermore, we show that Bcl-2 can protect cells from PCD in these anaerobic conditions. These results suggest that ROS are not required for PCD, and that Bcl-2 protects against PCD in ways that do not depend on the inhibition of ROS production or activity.

References

Aug 1, 1990·Bio/technology·T Tamaoki, H Nakano
Jan 1, 1990·Methods in Enzymology·B Halliwell, J M Gutteridge
Jan 1, 1989·Annual Review of Biochemistry·E Cadenas
Oct 22, 1993·Cell·D M HockenberyS J Korsmeyer
Mar 10, 1995·Science·S Nagata, P Golstein
Jan 1, 1994·Journal of Neurochemistry·R R RatanJ M Baraban
Jan 1, 1994·Immunology Today·T M Buttke, P A Sandstrom
Jan 1, 1994·The Journal of Cell Biology·J C Reed
Jan 28, 1993·Nature·M D JacobsonM C Raff
Apr 30, 1993·Biochemical and Biophysical Research Communications·M BaudryB Malfroy

❮ Previous
Next ❯

Citations

Aug 28, 1999·The Journal of Comparative Neurology·M NakamuraT K Mcintosh
May 16, 1997·International Journal of Cancer. Journal International Du Cancer·J M Jürgensmeier, G Bauer
Jun 9, 1999·International Journal of Cancer. Journal International Du Cancer·B PalazzottiT Galeotti
Apr 5, 1997·Biotechnology and Bioengineering·N H SimpsonM Al-Rubeai
Apr 15, 1997·Journal of Neuroscience Research·R W KeaneC Kayalar
Sep 19, 2000·Teratology·J P Kehrer
Dec 1, 1995·Annals of Neurology·D E Bredesen
Jul 12, 2002·Microscopy Research and Technique·Wolf D Splettstoesser, Peter Schuff-Werner
Mar 27, 2001·Biochemical and Biophysical Research Communications·M J Burkitt, P Wardman
Sep 1, 1995·Journal of Clinical Immunology·N Oyaizu, S Pahwa
Jan 1, 1996·Pathology Oncology Research : POR·Dean G Tang, Arthur T Porter
Aug 9, 2001·Irish Journal of Medical Science·R McLaughlinD Bouchier-Hayes
May 24, 2005·Apoptosis : an International Journal on Programmed Cell Death·S A MarshJ S Coombes
Feb 24, 2007·Apoptosis : an International Journal on Programmed Cell Death·Jeong-Yeh YangClifton A Baile
Sep 18, 2007·Apoptosis : an International Journal on Programmed Cell Death·Jeong-Yeh YangClifton A Baile
Jun 26, 2013·Journal of Natural Medicines·You-Hua XuQuan Zhu
Aug 1, 1996·European Journal of Obstetrics, Gynecology, and Reproductive Biology·D VinatierD Subtil
Oct 1, 1996·Neuroscience·N A SimonianJ T Coyle
Dec 1, 1996·Neuroscience·N A SimonianJ T Coyle
Dec 1, 1995·Toxicology Letters·A F SlaterS Orrenius
Oct 1, 1995·Current Opinion in Immunology·D R Green, S J Martin
Jan 1, 1996·Urologic Oncology·C N Coleman, M A Stevenson
Aug 15, 2013·Cancer Letters·Saravana R K MurthyY Peng Loh
Jun 20, 2002·Journal of Immunological Methods·James F CurtinThomas G Cotter
Sep 21, 2000·Mechanisms of Ageing and Development·W E MüllerH C Schröder
Sep 15, 2001·Mechanisms of Ageing and Development·M SasakiY Mitsui
May 21, 1998·Mechanisms of Ageing and Development·S PerovicW E Müller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.