Programmed cell death during microgenesis in a Honglian CMS line of rice is correlated with oxidative stress in mitochondria

Functional Plant Biology : FPB
Shaoqing LiYingguo Zhu

Abstract

The key role of mitochondria in the integration of apoptosis and oxidative stress in mammals has been documented. In plants, mitochondria are implicated in cytoplasmic male sterility (CMS), programmed cell death (PCD), and oxidative stress. However, to date there has been no evidence presented of the interplay among CMS, PCD and oxidative stress. In this study, we demonstrate that the pollen abortion of Honglian CMS line of rice (Oryza sativa L.) displays a PCD phenotype. A premature loss of microspores was accompanied by several biochemical markers of apoptosis. Analysis of mitochondria revealed that during the PCD process there was disruption of the inner mitochondrial membrane potential in microspores. This disruption was correlated with excess production of reactive oxygen species and down-regulation of the activity of superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase in mitochondria. The excess accumulation of ROS spanned three stages from pollen mother cell to early-uninucleate stage, and was followed by the occurrence of PCD at meiosis. Our data suggest that the microspores suffered from severe oxidative stress during pollen development. It is likely the chronic oxidative stress triggered the tissue-spe...Continue Reading

Citations

Jun 14, 2008·Yi chuan = Hereditas·Chao-Feng HuYing-Guo Zhu
Dec 14, 2011·Protoplasma·Ling HuangMoju Cao
Dec 10, 2013·Annual Review of Plant Biology·Letian Chen, Yao-Guang Liu
Jun 20, 2019·International Journal of Molecular Sciences·Xianlong DingShouping Yang
Jan 18, 2007·Plant Cell Reports·Hong ZhangYingguo Zhu
Sep 17, 2009·The Protein Journal·Qingping SunYingguo Zhu
Nov 9, 2014·Applied Biochemistry and Biotechnology·Jianxin LiQunce Huang
Dec 17, 2020·International Journal of Molecular Sciences·Maria Breygina, Ekaterina Klimenko
Nov 9, 2005·Mitochondrion·Bettina Linke, Thomas Börner

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