PMID: 8945735Nov 1, 1996Paper

Programmed cell death in rat microglia is controlled by extracellular adenosine

Neuroscience Letters
T Ogata, P Schubert

Abstract

The induction of programmed cell death by adenosine was investigated in cultured rat microglial cells using the enzyme-linked immunosorbent assay (ELISA) for determining DNA fragmentation. Twelve hours exposure to micromolar levels of the unselective adenosine receptor agonist 2-chloro-adenosine led to the appearance of DNA fragments in the cytosolic fraction preceding damage of the plasma membrane. This effect was still seen in the presence of an adenosine uptake blocker. Conventional A1, A2 or A3 agonists and antagonists were rather ineffective, suggesting mediation via an atypical adenosine receptor subtype. Microglial DNA fragmentation was inhibited by H-7 and staurosporine but not by dibutyryl-cyclic AMP, pointing to a protein kinase C linked mechanism. Such an induction of programmed cell death by an elevation of the extracellular adenosine concentration may provide an endogenous control mechanism to limit the function of activated microglial cells.

Citations

Apr 12, 2008·Purinergic Signalling·Beáta Sperlágh, Peter Illes
May 29, 1998·Progress in Neurobiology·H Aldskogius, E N Kozlova
Dec 11, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Miao ChuaiPeter Schubert
Nov 25, 1997·Annals of the New York Academy of Sciences·P SchubertK Rudolphi
May 20, 2000·Annals of the New York Academy of Sciences·P SchubertS Ferroni
Aug 21, 2002·Biological & Pharmaceutical Bulletin·Yoichi Nakamura
Jul 21, 2007·Neurochemistry International·Zsolt SelmeczyGyörgy Haskó
Feb 22, 2003·The Journal of Comparative Neurology·Ishar DalmauBernardo Castellano
Dec 13, 2005·Neurochemical Research·Dietrich van Calker, Knut Biber
Apr 30, 2011·Physiological Reviews·Helmut KettenmannAlexei Verkhratsky
Oct 31, 2002·Reviews in the Neurosciences·Elisabetta Polazzi, Antonio Contestabile
Sep 5, 2006·Cancer Research·Michael SynowitzHelmut Kettenmann

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